发酵葡萄籽中原花青素的提取与分离.研究

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1、郑州大学硕士学位论文Abst扭CtTheProPerties，effieaey，andPharmacologicaleffeetsofProanthoeyanidinsineludingtheProgressofthestudyathomeandabroadareintrodueedinthisthesis.ThedissertationmainlystudiedtheteehnologyofextractionandseParationfromthefermentedgraPeseed.Theex

2、tractioneonditions，seParationmethodandseParationeonditionsofProanthoeyanidinsfromgraPeseedseanbeobtainedbyexPeriment.ThemainworkofthethesisandachievementaresummeduPasfollows:1.BasedonthePreviousresults，thevanillin一hydrochlorieacidmethodwasfoundedforana

3、lyzingProanthocyanidins.UnearrelationandtheregressionequationweremakedbetweeneoneentrationandabsorbaneewiththestandardsamPle.2.InextraetionProeessfromthefermentedgraPe，ethanolsolventwasmailyusedinthisPaPer，fiveextractionconditionswerestudied:theextract

4、iontimes，extractiontime，temPerature，concentrationofsolventandratioofsolidtoliquid.TheresultshowedthattheoPtimumextrationconditionswereobtainedasfollows:eoneentrationofethanolat60%，ratioofsolidtoliquidatl:7，temPeratureat58，C，extraetionfor3timesand30minu

5、teseachtime.Theextractionyield151.58%withthismethod.Inaddition，mixedsolventsofmethylaleohol，ethanolandacetoneeffectontheextraetionratewasstudied，theresultsshowedthattheextractionratewas2.06%whenthesolventProPortionwasl:3:2，theextractionrateofthemixedso

6、lventswas28.0%higherthanthatofsinglesolvent.3.TheoPtimalexPerimentconditionswereachievedbyorthogonalexPeriment:aecordingtotheirsignificance，thedescendingsequeneeofabovefoactorsWasB(temPerature)>D(extractiontime)>C(aleoholvolumefraetion)>A(thesolid一liqu

7、idratio).TheoPtimizationProcessconditionswereAZB3CID3，withthetemPeratureof60oC，fromtimeto40min，alcoholvolumefractionof50%andthesolid一liquidratioatl:7.Andthebetterextractionrateeanreaehto1.61%.4.MacroPorousresinwasusedintheseParationofProeyanidins.After

8、studyingsixmaeroPorousadsorptionresinswithcolumnchromatograPhy，03F3resinwasseleetedasthebestadsorbent，thedynamiestateadsorptionProPertiesanddesorptionProPertiesofO3F3resinwerestudied.ExPerimentsresultsindieatedasfollows:03F3resinwasanex