尼可地尔改善心脏停搏液对心脏的保护作用

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1、尼可地尔改善心脏停搏液对心脏的保护作用作者：史红宇，朱晓峰，战鹏，温福兴，龙村【关键词】心肌保护摘要：目的观察在HTK液和UW液中加入尼可地尔（Nicorandil）对心肌保存液保存效果的影响。方法实验分为4组：UW液（U）组；HTK液（H）组；UW液组+Nicorandil（U+N）组；HTK液+Nicorandil（H+N）组；每组8只大白鼠。麻醉和抗凝后，快速取下鼠心并悬挂在Langendorff灌注模型上灌注，测定血流动力学基础值。分别用四组保存液灌停离体鼠心并低温（4℃）浸泡保存6h。复温、复灌后再次测定血流动力学值，留取标本分别测定心肌水含量、心肌酶漏

2、出量、心肌细胞ATP和CP含量和心肌细胞超微结构变化。结果心肌血流动力学恢复率：H+N组＞U+N组＞H组＞U组。心肌水含量：4组间差异无显著意义。灌脉流出液中心肌酶漏出量：H+N组＜H组＜U+N组＜U组。心肌中ATP和CP含量：H+N组＞U+N组＞H组＞U组。心肌细胞超微结构变化：H+N组心肌损害最轻，H组轻于U+N组，U组心肌损害最严重。结论HTK液和UW液都是比较合适的心肌保存液，HTK液对心肌的保护作用优于UW液，钾离子通道开放剂Nicorandil能够善器官保存液HTK液和UW液对心肌的保存效果。　　关键词：HTK液；UW液；尼可地尔；心肌保护12　　Th

3、eMyocardialPreservationEffectofNicorandilinHTKSolutionandUWSolution　　Abstract:OBJECTIVEWeinvestigatedtheefficacyofHTKsolutionandUWsolutionwithNicorandil（NCR）intheisolatedratheartmodels.METHODSTheheartsweredividedintofourgroups:UWsolution（U）group;HTKsolution（H）group;UWsolutionwithNicor

4、andil（U+N）group;HTKsolutionwithNicorandil（H+N）group.TheheartsisolatedfromWistarratsweremountedonaLangendorffapparatustoestimatebaselinehemodynamicvalues.Theywerearrestedandstoredat4℃ineachpreservationsolutionfor6hours.Therecoveryofmyocardialfunctionweremeasured.Myocardialtissuewaterco

5、ntentwasevaluated.MyocardialtissueAdenosineTriphosphate(ATP)andCreatinePhosphate(CP)contentineachgroupwerealsomeasured.Theleakageofmyocardialenzymesweremeasuredaswell.Finally,myocardialultrastructurewasobserved.RESULTSTherecoveryrateofmyocardialfunction:thegroupofH+N>thegroupofU+N&

6、gt;thegroupofH>thegroupofU.Themyocardialtissuewatercontent:therewasnosignificantchangesinfourgroups;Theleakageofmyocardialenzymes:thegroupofH+N<the12groupofH<thegroupofU+N<thegroupofU.ThelevelofATPandCPinmyocardialtissue:thegroupofH+N>thegroupofU+N>thegroupofH>the

7、groupofU.Themyocardialultrastructure:ThehistologicalchangesofmyocardialcellandthedamageofvascularendothelialcellweremoresignificantinU+NgroupcomparedwiththatinH+Ngroup.ThehistologicalchangesweresevererinUgroupthanthatinU+Ngroup.CONCLUSIONTheseresultssuggestthatHTKsolutionismoreeffecti

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