菊花多糖不同提取工艺研究.pdf

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1、28粮食与油脂2014年第27卷第7期菊花多糖不同提取工艺研究陈云，李文治，罗其昌，高群玉(1．华南理工大学轻工与食品学院，广东广州510640；2．无限极有限公司，广东江门529000)摘要：以菊花(产自杭州的杭白菊)为材料，比较水浸提法、超声波辅助提取法和酶辅助提取法对菊花多糖得率的影响，得出最优提取方法和提取条件。考察了水浸提法中提取时间、料液比和提取温度对多糖得率的影响，得出最优水提工艺为提取时间2h、料液比1：25(W／v)、提取温度90oC；考察了超声波辅助提取法中提取时间、料液比、提取温度和功率对多糖得率的影

2、响，得到最优条件为提取时间35min、料液比1：25、提取温度70℃、提取功率60W；考察了酶提取法中纤维素酶水解提取菊花多糖提取时间、料液比和酶用量对多糖得率的影响，得出的最优条件为提取时间2h、物料比l：40、加酶量占样品重的2％。结果表明，在所设定的实验条件下菊花多糖的得率，以酶提取法最高，超声波辅助提取法次之，水浸提法最低。关键词：菊花；多糖；水浸提法；超声波辅助法；酶法Studyondiarentextractionprocess0fp0lVsaccharidesfromchrysanthemumCHENYun，

3、LIWen-zhi，LUOQi-chang，GAoQun-yu(1．CollegeofLightIndustryandFoodScience，SouthChinaUniversityofTechnology，Guangzhou510640，Guangdong，China；2．InfintusLtd．，Jiangmen529000，Guangdong，China)Abstract：Threedifferentproceduresofhotwater，ultrasonic—assistedandenzymefortheextr

4、actionofpolysaccharidefromChrysanthemumwerestudied．Thethreeprocedureswerecomparedandthenthebestprocedureandtheoptimumextractionconditionswereobtained．Thehotwaterextractionconditionsofchrysanthemumpolysaccharideswereinvestigated．Threeparametersafectingtheextraction

5、eficiencyofpolysaccharideswereextractiontimes，ratioofwatertoraWmaterialandextractiontemperature．Theoptimumextractionconditionswerefoundtobeextractiontime2h．ratioofwatertoraWmaterial25(W／V)．extractiontemperature90℃．11】eparametersofultrasonic—assistedexVactiononextr

6、actioneficiencyofpolysaccharideswereextractiontime，ratioofwatertoraWmaterial，extractiontemperatureandultrasonicpowerandtheoptimumextractionconditionsofultrasonic-assistedextractionwereextractiontime35min，ratioofwatertoraWmaterial25，extractiontemperature70oCandultr

7、asonicpower60W．Thecellulaseextractionconditionsofchrysanthemumpolysaccharideswereinvestigatedtoo．Threeparametersafectingtheextractioneficiencyofpolysaccharideswereextractiontime，ratioofwatertorawmaterialandtheratioofcelluasetorawmateria1．Theoptimumconditionsofcell

8、uaseextractionwereextractionlime2h，ratioofwatertoraWmaterial40andtheratioofcelluasetoraWmaterial0．02．Astheresult．thebestprocedurefortheextractionofpolys