纤溶豆豉的制备及其对小鼠溶栓效果的评价-论文.pdf

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1、2282012，Vo1．33,No．03目品科学※营养卫生纤溶豆豉的制备及其对小鼠溶栓效果的评价李小东，王成涛，赵磊，杨雪莲，张佳婵(北京工商大学食品添加剂与配料北京高校工程研究中心，食品风味化学北京市重点实验室，北京100048)摘要：利用动物血栓模型，饲喂纤溶豆豉来研究其在动物体内的溶栓作用。通过小鼠腰背部皮下注射l0mg／mL角又菜胶溶液诱发小鼠尾静脉形成血栓，实验组A饲喂豆豉纤溶豆豉并自由饮用纤溶豆豉提取液，对照组B(模型小鼠)、对照组c(正常小鼠)分别饲喂全价营养鼠粮，并自由饮水。结果

2、表明：各组小鼠生长情况良好；饲喂纤溶豆豉的实验组A小鼠体质量先降低后增高，饲喂5d以后尾部形成的血栓长度逐渐变短，1Od时凝血酶原时间、凝血酶时间稍有延长，但差异不显著，15d后凝血酶原时间、凝血酶时间均明显延长(P

3、血栓长度；纤溶活性；角叉菜胶PreparationofFibrinolyticDouchiandEvaluationofItsThrombolyticEffectivenessinvivoLIXiao—dong，WANGCheng—tao，ZHAOLei，YANGXue—lian，ZHANGJia—chan(BeijingHigherInstitutionEngineeringResearchCenterofFoodAdditivesandIngredients，BeijingKeyLabora

4、toryofFlavorChemistry，BeijingTechnologyandBusinessUniversity，Beijing100048，China)Abstract：TheefectoffeedingDouchiwasevaluatedonmousethrombosisinducedbysubcutaneous．mjectionwith10mg／mLcarrageenanatthebackwaistofmice．ICRmiceweredividedintothreegroups：D

5、ouchitreatmentgroup(thrombosismodelmicefedDouchiandnormalsalineextractfromDouchi)，modelcontrolgroup(thrombosismodelmicefedfullnutritiondietanddistilledwater)andnormalcontrolgroup(normalmicefedfullnutritiondietanddistilledwater)．Theresultsshowedthatth

6、emiceintheDouchitreatmentgroupexhibitedallinitialdecreaseandsubsequentincreaseinbodyweight，astightincreaseinprothrombintime(PandthrombintimeT)，andareductioninplasmafibrinogen(FiB)duringtheexpedmenLThedetectionofD-dimerwaspositiveafter10daysoffeedinga

7、ndPTand1Twerenotablyprolonged(P<0．05)inthemicefedfor15days．However,PTandTrshowednosignificantdifferencebetweenthemodelcontrolgroupandnormalcontrolgroup．Therefore，DouchiCanactivamthefunctionoffibrinolysis，inhibitthefunctionofblood-clottingsystem，andpr

8、eventandtreatthrombosisdiseases．Keywords：ChineseDouchi；thrombosismodel；lengthofinfarctedregions；fibrinolyticactivity；carrageenan中图分类号：TS201．4文献标识码：A文章编号：1002．6630(2012)03．0228—04豆豉是我国的传统发酵食品，营养丰富、风味独张炳文等[31也发现豆豉激酶对角叉菜胶造成的小鼠血栓特，并且具有开胃增加食欲及预防心脑血管疾病的功效形