二苯乙烯苷对D 半乳糖致拟痴呆小鼠学习记忆和海马APP、Aβ表达的影响【大学临床医学毕.doc

《二苯乙烯苷对D 半乳糖致拟痴呆小鼠学习记忆和海马APP、Aβ表达的影响【大学临床医学毕.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、临床医学论文■•二苯乙烯昔对D・半乳糖致拟痴呆小鼠学习记忆和海马APP、AB表达的影响作者：黄忠仕，谢海源，农嵩，李韬，张树球【摘要】目的观察二苯乙烯昔(TSG)对D・半乳糖致拟痴呆小鼠学习记忆能力及海马B淀粉样蛋白前体(APP)、/3淀粉样蛋白(A/3)表达的影响。方法连续给昆明种小鼠灌胃TSG50天，同时每天颈部皮下注射D・半乳糖建立痴呆小鼠模型，以Morris水迷宫为学习记忆评价指标，并采用免疫组化及图像分析测定各组小鼠海马组织APP、AB平均光密度和阳性细胞数。结果在隐蔽平台试验中拟痴呆小鼠表现出明显的学习记忆障碍，逃避潜伏期显

2、著延长(P<0・05)，TSG各剂量组逃避潜伏期较模型组显著缩短(P<0.05)；探索试验屮TSG各剂量组原平台象限的停留时间和穿越原平台次数明显多于模型组。与模型组比较，TSG各剂量组能降低拟痴呆小鼠脑内APP、A/3平均光密度値和阳性细胞数(PvO.05)。以上效应均呈现一定的量效关系。结论TSG各剂量能明显地改善拟痴呆小鼠的学习记忆能力，能减少或抑制APP、A/3的过度产生，保护神经元免受AB的神经毒性。【关键词】二苯乙烯昔痴呆学习记忆能力淀粉样B蛋白前体淀粉样B蛋白小鼠动物实验Abstract:ObjectiveTostudyt

3、heeffectsof2,3,5,tetrahydroxy・stiIbeneD-glylcoside(TSG)onlearningandmemoryabi1itiesandexpressionsofB・amyloidprecursorprotein(APP)andB・amyloid(A/3)inhippocampusofmicewithdementiainducedbyD・galactose.MethodsAfterthemiceweretreatedwithintragastricadministrationwithTSGfor50d

4、ayscontinually,themiceweretreatedsubcutaneousinjcctionwithDalactoscatthesametimctoeslablishthedementiamodel,thentheMorriswatermazelestwasperformedtosurveythelearningandmemoryabilities,andtheimmunohistochemicalanalysisandcomputerizedimagepresentationanalysissystemwereused

5、todeterminetheAPPandA/3averageopticaldensityandtheirimmunorcactivcneuronsinhippocampus.ResultsThemicewithdementiahadlearningandmemorydisordersduringhiddenplatformtest,andtheescapelatencyweresignificantlylongerthanthatofTSG・treatedgroups(Pv0・05)・TSG・treatedgroupscouldsign

6、ificantlyprolongthestayingtimeandthefrequencyofcrossingoriginalplatformquadrantinspatialprobetest(P<0・05)・Comparedwithmodelgroup,TSG-treatedgroupscoulddecreasesignificantlytheAPPandA/3averageopticaldensityandtheirimmunoreactiveneuronsinhippocampusofmicewithdementiaviaimm

7、unohistochemicalanalysis(P<0.05).Allthedatashowedadose-responserelationshiptosomedegree・ConclusionTSGcanimprovethelearningandmemoryabilitiesinmicewithdementiaandreduceorinhibittheover-expressionofAPPandA/3inhippocampusofmicewithdementia,soastoprotecttheneuronsfromtheneur

8、otoxicityofA/3.Keywords:2,3,5,4'・tetrahydroxy・stilbcnc・2・O・/S・D・glylcosidc;dementia;learningandmemoryab