二苯乙烯苷对d-半乳糖致拟痴呆小鼠学习记忆和海马app、aβ表达的影响

《二苯乙烯苷对d-半乳糖致拟痴呆小鼠学习记忆和海马app、aβ表达的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、二苯乙烯苷对D-半乳糖致拟痴呆小鼠学习记忆和海马APP、Aβ表达的影响作者：黄忠仕，谢海源，农嵩，李韬，张树球【摘要】　　目的观察二苯乙烯苷（TSG）对D-半乳糖致拟痴呆小鼠学习记忆能力及海马β淀粉样蛋白前体(APP)、β淀粉样蛋白(Aβ)表达的影响。方法连续给昆明种小鼠灌胃TSG50天，同时每天颈部皮下注射D-半乳糖建立痴呆小鼠模型，以Morris水迷宫为学习记忆评价指标，并采用免疫组化及图像分析测定各组小鼠海马组织APP、Aβ平均光密度和阳性细胞数。结果在隐蔽平台试验中拟痴呆小鼠表现出明显的学习记忆障碍，逃避潜伏期显著延长（P<0.05），TSG各剂量组逃避潜伏期较模型

2、组显著缩短（P<0.05）；探索试验中TSG各剂量组原平台象限的停留时间和穿越原平台次数明显多于模型组。与模型组比较，TSG各剂量组能降低拟痴呆小鼠脑内APP、Aβ平均光密度值和阳性细胞数(P<0.05)。以上效应均呈现一定的量效关系。结论TSG各剂量能明显地改善拟痴呆小鼠的学习记忆能力，能减少或抑制APP、Aβ的过度产生，保护神经元免受Aβ的神经毒性。【关键词】二苯乙烯苷痴呆学习记忆能力淀粉样β蛋白前体淀粉样β蛋白小鼠动物实验　　Abstract:ObjectiveTostudytheeffectsof2,3,5,144'-tetrahydroxy-stilbene

3、-2-O-β-D-glylcoside(TSG)onlearningandmemoryabilitiesandexpressionsofβ-amyloidprecursorprotein(APP)andβ-amyloid(Aβ)inhippocampusofmicewithdementiainducedbyD-galactose.MethodsAfterthemiceweretreatedwithintragastricadministrationwithTSGfor50dayscontinually,themiceweretreatedsubcutaneousinjection

4、withD-galactoseatthesametimetoestablishthedementiamodel,thentheMorriswatermazetestwasperformedtosurveythelearningandmemoryabilities,andtheimmunohistochemicalanalysisandcomputerizedimagepresentationanalysissystemwereusedtodeterminetheAPPandAβaverageopticaldensityandtheirimmunoreactiveneuronsin

5、hippocampus.ResultsThemicewithdementiahadlearningandmemorydisordersduringhiddenplatformtest,andtheescapelatencyweresignificantlylongerthanthatofTSG-treatedgroups(P<0.05).TSG-treatedgroupscouldsignificantlyprolongthestayingtimeandthefrequencyofcrossingoriginalplatformquadrantinspatialprobet

6、est(P<0.05).Comparedwithmodelgroup,TSG-treatedgroupscoulddecreasesignificantlytheAPPandAβaverageopticaldensityandtheirimmunoreactiveneuronsinhippocampusofmicewithdementiaviaimmunohistochemicalanalysis(P<0.05).Allthedatashowed14adose-responserelationshiptosomedegree.ConclusionTSGcanimpro

7、vethelearningandmemoryabilitiesinmicewithdementiaandreduceorinhibittheover-expressionofAPPandAβinhippocampusofmicewithdementia,soastoprotecttheneuronsfromtheneurotoxicityofAβ.　　Keywords:2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glylcoside;dement