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1、_ArchivesArchVirol(1995)140:1303-1311Virology©Springer-Verlag1995PrintedinAustriaAntigenicandbiologicalcomparisonsofbovinecoronavirusesderivedfromneonatalcalfdiarrheaandwinterdysenteryofadultcattleBriefReportH.TsunemitsuandL.J.SaffFoodAnimalHealthResearchProgram,OhioAgricultural
2、ResearchandDevelopmentCenter,TheOhioStateUniversity,Wooster,Ohio,U.S.A.AcceptedFebruary24,1995Summary.Theantigenicandbiologicalpropertiesof6strainsofbovinecorona-virus(BCV)derivedfromneonatalcalfdiarrhea(CD)and8strainsofBCVfromwinterdysentery(WD)ofadultcattle,propagatedinHRT-18c
3、ells,werecomparedtodetermineifCDandWDstrainsbelongtodistinctserotypesorsubtypesofBCV.Allstrainshemagglutinatedbothmouseandchickenerythrocytesat4°C,buttheratiosofhemagglutinationtiterswithmouseerythrocytescomparedtochickenerythrocytesshoweddiversityforbothCDandWDstrains.SomeCDand
4、WDstrainsdidnothemagglutinatechickenerythrocytesat37°Candshowedreceptor-destroyingenzymeactivityagainstchickenerythrocytes.Hyperimmuneantiserawereproducedinguineapigsagainst3and7strainsofBCVfromCDandWD,respectively.Nosignificantdifferencesinantibodytitersagainstthesestrainswereo
5、bservedbyindirectimmunofluorescencetests.However,invirusneutralizationtests,antiserato1CDand2WDstrainshad16-foldorlowerantibodytitersagainst3WDand1CDstrainsthanagainstthehomologousstrains,andthisvariationreflectedlowantigenicrelatednessvalues(R=13-25%),suggestingthepresenceofdif
6、ferentsubtypesamongBCV.Inhemagglutinationinhibitiontests,someone-wayantigenicvariationsamongstrainswerealsoobserved.TheseresultssuggestthatsomeantigenicandbiologicaldiversityexistsamongBCVstrains,butthesevariationswereunrelatedtotheclinicalsourceofthestrains;i.e.CDorWD.Bovinecor
7、onavirus(BCV)isaprimarycauseofneonatalcalfdiarrhea(CD)worldwide[4,13].Bovinecoronavirusisalsoassociatedwithacutediarrheainadultcattleduringthewinterseasonreferredtoaswinterdysentery(WD)[4,17,18,24,26].Besidesinfectingthesmallandlargeintestinesofcalves,BCValsopossessesatissuetrop
8、ismfortheupperrespiratorytract[15].1304H.Tsunem
