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1、Eur.J.Epidemiol.0392-2990Vol.2,No.2June1986,p.112-117EUROPEANJOURNALOFEPIDEMIOLOGYPREVALENCEOFANTIBODYTOHUMANCORONAVIRUSES229E,OC43ANDNEONATALCALFDIARRHEA.CORONAVIRUS(NCDCV)INPATIENTSOFNORTHERNITALYP.M.CEREDA1L.PAGANIE.ROMEROInstituteofMicrobiology,UniversityofPavia,27100Pavia,Ita
2、ly.Keywords:Hnnaancoronaviruses-229E-OC43-BovinecoronavirusNCDCV.AseroepidemiologicalstudyfordetectionofantibodytohatmancoronavirusesOC43,229E,andneonatalcalfdiarrheacoronavirus(NCDCV),hasbeencarriedoutusingseracollectedfromhospitalizedpatientsorhealthypersonsthroughroutinelaborat
3、orytestsinNorthernItaly.Patientstestedwerechildrenandadultswithdifferentpathologicaldiseases.Antibodydetectionwasperformedbyusinganindirectimmunoperoxidasestainingtechnique(gorallviruses)and,inthecaseofOC43andNCDCV,antibodydetectionwasobtainedevenwitha,hemagglutinationinhibitionte
4、standaplaquereductionneutralizationassay.Resultsobtainedshowasignificantdifferenceintheprevalenceofantibodyto229Ebet~Teenchildrenandadultgroup.Furthermore,adifferenttirerwasobserved,withinthetwogroups,betweenpatientsaffectedbyhematologicaldiseases(leukemia)andpatientsw&hotherdisea
5、ses.Finally,ourdataseemtoconfirmpreviousstudiesreportingaveryhighprevalenceofantibodytocoronavirusOC43butalessdetectableseropositivitymcoronavirus229E.INTRODUCTIONii)morphologyshowingpleomorphicenve-lopedparticleswithdiameterrangingfrom80toHumancoronavirusesOC43and229Earewell160nm
6、andsurfaceprojections(spikes)pro.trudingknownasetiologicalagentsofrespiratoryinfec-12-24nmfromenvelope;tionsgenerallyaffectingtheupperrespiratoryiii)commonphysicochemicaipropertiessuchtractandresponsibleforcommoncoldsduringasbandingat1.1.6.-1.23g/cm3insucrosegradients,winter-sprin
7、gseasonsIsolationofthesevirusesdisruptionbvether,chloroform,anddetergents,isparticularlydifficultand,consequently,allremovalofsurfaceprojectionsbybromelainandepidemiologicalandreliabledataabouttheirtrypsin.diffusionarebaseduponserologicalstudiesConsequently,virusescouldbeincludedi
8、ntheperformedusingreferencestrain
