冠状病毒Coronavirusl论文-1999 Detection of bovine coronaviruses from adult cows with epizootic diarrhea and their antigenic and biological divers.pdf

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1、ArchVirol(1999)144:997–1006DetectionofbovinecoronavirusesfromadultcowswithepizooticdiarrheaandtheirantigenicandbiologicaldiversitiesBriefReportT.Fukutomi1,H.Tsunemitsu2,andH.Akashi31OkayamaPrefecturalVeterinaryDiagnosticLaboratory,Mitsu-cho,Okayama,Japan2ShichinoheResearchUnit,NationalInstitu

2、teofAnimalHealth,Shichinohe,Aomori,Japan3NationalInstituteofAnimalHealth,Kannondai,Tsukuba,Ibaraki,JapanAcceptedDecember17,1998Summary.Bovinecoronavirus(BCV)wasdetectedbyreversetranscriptase-PCR,immuneelectronmicroscopyorvirusisolationfromadultcowsat6outof6outbreaksofepizooticdiarrheainJapan.

3、SixBCVsisolatedinfeces,intestinalcontentortrachealexudateofthecowswereanalyzedfortheirantigenicproper-tiesbycrossvirusneutralization(VN)tests.Theisolatesweredividedintotwogroups,oneofwhichhadcloselyrelatedantigenicitywiththereferenceMebusandKakegawastrainsofBCV,andanotherwhichshowedsignificant

4、differencesinVNantibodytitersfromthereferencestrains.Twoisolatesinthelattergroup,whichwerefromtheentericandrespiratorytractsofthesamecows,respec-tively,weredistinguishedfromeachotherbyELISAusingmonoclonalantibod-iesagainsttheKakegawastrain.Theisolatesshowedvarioushemagglutinationandreceptorde

5、stroyingenzymetitersagainstchickenormouseerythrocytes.Bovinecoronavirus(BCV)belongstoantigenicgroup2oftheCoronaviridae,andpossessesfourmajorstructuralproteins,thenucleocapsidproteinN,theintegralmembraneglycoprotein(gp)M,thehemagglutinin-esterasegpHEandthespikegpS[4].ItisknownthatbothHEandSgp

6、sareinvolvedinhemagglutination(HA)activity.TheHEgpisalesspotenthemagglutininthantheSgp,asitonlyagglutinateserythrocytesfrommiceandratsandnotthosefromadultchickens.TheHEgpalsoexhibitsreceptordestroyingenzyme(RDE)activity[4].BCVisknownasamajorcausativeagentofcalfdiarrhea[3,4,10,15,24,25]andwint

7、erdysenteryofadultcattle[2,4,8,18,28,30].Itstillremainsdifficulttoisolatethevirusoncellcultures.Thus,therehavebeenfewpapersontheisolation998T.Fukutomietal.andantigenicityofBCV,despitenumerousreportsontheirmolecularnature.Thepurposeofthisstudyw