冠状病毒Coronavirusl论文-1999 Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected.pdf

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1、ArchVirol(1999)144:2441–2447Antigenicvariationamongbovineentericcoronaviruses(BECV)andbovinerespiratorycoronaviruses(BRCV)detectedusingmonoclonalantibodiesBriefReportM.Hasoksuz,S.Lathrop,M.A.Al-dubaib,P.Lewis,andL.J.SaifFoodAnimalHealthResearchProgram,DepartmentofVeterinaryPreventiveMedicine,O

2、hioAgriculturalResearchandDevelopmentCenter,TheOhioStateUniversity,Wooster,Ohio,U.S.AAcceptedJuly22,1999Summary.Bovinecoronavirus(BCV)causesneonatalcalfdiarrhea(CD)andisassociatedwithwinterdysentery(WD)inadultdairycattle.Itcanalsobeiso-latedfromtherespiratorytractsofcattleenteringfeedlots.Monoclon

3、alantibodies(MAbs)specificforthehemagglutininesterase(HE)andspike(S)surfacepro-teinsof2bovineentericcoronavirus(BECV)strainsandtwobovinerespiratorycoronavirus(BRCV)strainsweretestedagainst6BECVstrainsand6recentlyisolatedBRCVstrains,inordertocharacterizetheantigenicityofBCVstrainswithvariedtissuetro

4、pisms.AllMAbshadhighimmunofluorescence(IF)titersagainstBECVandBRCVstrains,indicativeofconservedcross-reactiveepitopes.Inhemagglutinationinhibition(HI)tests,theS-MAbsweremorebroadlyreactivethanHE-MAbs.TheBRCVandCDMAbsweremorebroadlyreactiveinHIthantheWDMAbs.TheHAactivityoftheMebusvaccineCDstrainwasn

5、otinhibitedbyanyoftheMAbstested.TheHIactivityofBRCVstrainR6wasuniqueamongthe6BRCVisolates.Invirusneutralizationassays,MAbstotheBRCVstrainR4neutralizedall6BECVstrainstested.AntigenicvariationexistsamongbothBECVandBRCVstrains,butitcannotbeattributedsoleytotheclinicaloriginofthestrain.Permanentaddr

6、ess:VeterinaryFaculty,DepartmentofMicrobiology,IstanbulUniver-sity,Istanbul,Turkey;CollegeofAgricultureandVeterinaryMedicine,KingSaudUniver-sity,Burydah,SaudiArabia.2442M.Hasoksuzetal.Table1.Hemagglutinationinhibition(HI)reactivityofselectedmonoclonalantibodieswithBECVandBRCVstrainsMAbVPaIsotype

7、CalfdiarrheaWinterdysenteryFeedlotBECVBECVBRCVDB2MebusSDCDBABMBER1R2R3R4R5R6DB-2MAbs(CD)CD-1NDbIgG2bCc−c−−C−CCC−−−CD-2SIgG2aC−−−C−CC−−−−DBAMAbs(WD)WD-1SIgG2aC−−C−−CCC−−−WD-2HEIgG1−−−C−−−−−−−−WD-3HEIgA−−−C−−−−−−−−