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1、福建医科大学2011届硕士研究生学位论文RoleofhighthoracicepiduralanesthesiaplusnimodipineintherabbitmodelofcerebralvasospasmfollowingsubarachnoidhemorrhageAbstractOBJECTIVETocomparetheefficacyofhighthoracicepiduralanesthesia(HTEA)andnimodipineinpreventingcerebralvasospasm(CVS)followi
2、ngsubarachnoidhemorrhage(SAH)inrabbits.Andtoinvestigatetheroleofhighthoracicepiduralblockplusnimodipineintherabbitmodelofcerebralvasospasmfollowingsubarachnoidhemorrhage.METHODSTheHTEAmodelatT6～7epiduralspacewasestablishedin90rabbits,whowererandomlyassignedto5group
3、sof6rabbitseach,i.e.thesham-operatedgroup(S),Controlgroup(Con),HTEAtreatmentgroup(HS),Nimodipinetreatmentgroup(NS)andHTEAplusNimodipinetreatmentgroup（HNS）.SAHwasdevelopedbytwoinjectionofautobloodintothecisternmagna(0.5ml/kg),TranscranialDopplerultrasonographywasuse
4、dtodetectthebasilararterymeanflowvelocity(Vm)1,7and14daysafterSAH.SerumS100BandNSEconcentrationisdeterminedbyELISAin1，7，14daysafterSAH.ThechangesinhistologicalmorphologyofthebasilararterywereobservedunderalightMicroscope,andTUNELstainingwereconductedonbasilararteri
5、escollected,Bcl-2andCaspase-3weremeasuredintheendothelialcellsofbasillaryarterybyimmunohistochemistry.RESULTSComparedtoSgroup,TheVmandVpofthebasilarartery,SerumS100BandNSEwerefoundtobeelevated,theluminaofthebasilararteriesbecamenarrowandthewallsofthebasilararterywe
6、rethick(P<0.05);TheVmandVpofthebasilarartery,SerumS100BandNSEinthe7福建医科大学2011届硕士研究生学位论文controlgroupwassignificantlyfasterthanthatinthetreatmentgroups(P<0.05);TherewereinsignificantdifferenceintheVmandVpofthebasilararteryandSerumS100BandNSEamongthetreatmentgroups.Pa
7、thologicalobservationsshowedthatinthecontrolgrouptheluminaofthebasilararterieswasnarrowerandthebasilararterywallswerethickerthanthoseinthethreetreatmentgroups,Moreover,theapoptosisindexofendothelialcellswasalsosignificantlyincreased，Bcl-2weredown-regulatedandCaspas
8、e-3wereup-regulatedinothergroups;buttherewerenosignificantdifferencebetweentheNimodipineandHTEAtreatmentgroups，AndComparedtoCon,NSandHSgroup,ther
