ec-sod在大鼠肝脏缺血再灌注损伤中的表达变化

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1、授予单位代码10089学号或申请号i3852HebeiMedicalUniversity硕士学位论文在职科学学位EC-SOD在大鼠肝脏缺血再灌注损伤中的表达变化学位申请人：聂亚楠导师：王磊副教授专业：人体解剖与组织胚胎学二级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权

2、均归河北医科大学所有。否则,承担相应法律责任。研究生签名•本D导师签章：二级学院领导盖章：年《月S/日/河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写,文责自负卩研究生签名淡4衅导师签章：；>0-年月、丨曰目录中文摘要·············································································1英文摘要·············

3、································································5研究论文EC-SOD在大鼠肝脏缺血再灌注损伤中的表达变化前言·············································································10材料与方法····································································11结果···································

4、··········································19附图·············································································20附表·············································································27讨论·············································································2

5、9结论·············································································30参考文献·······································································30综述氧化应激、促氧化剂与抗氧化剂的研究进展·······················33致谢···············································································

6、····44个人简历·············································································45中文摘要EC-SOD在大鼠肝脏缺血再灌注损伤中的表达变化摘要临床上在进行肝脏手术如肝脏部分切除、肝脏移植以及处理严重肝脏外伤时往往需要暂时性阻断肝脏血流一段时间后再重新恢复其血液供应。在这个过程中就造成了肝脏的缺血再灌注损伤（hepaticischemiareperfusioninjury，HIRI）。HIRI是肝脏外科手术过程中一种常见的病理生理现象。肝脏的缺血再灌注可引起严重的肝细胞损伤

7、和肝功能障碍，这也是导致术后肝功能衰竭和病人预后较差的重要原因之一。研究已证实，导致这种损伤的主要机制之一为氧化应激反应。缺血再灌注时机体会产生大量活性氧自由基(reac-tiveoxygenspecies,ROS)。ROS包括超氧阴离子-(O2)、羟自由基(OH)和过氧化氢(H2O2)等。ROS的化学性质非常活泼，可以破坏细胞内的蛋白质、DNA和脂类等生物大分子，引起细胞死亡。超氧化物歧化酶（SuperoxideDismutases，SOD）是机体内一种重要--的抗氧化酶，负责清除O2，它能将O2转化成H2O2和氧，在机体抗氧化应激反应中具有重要作用。在哺乳动