x线对胶质瘤细胞生长和放射敏感性相关基因表达的影响与mgr2细胞株放射抗拒性的诱导

《x线对胶质瘤细胞生长和放射敏感性相关基因表达的影响与mgr2细胞株放射抗拒性的诱导》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、◇中山大学x线对胶质瘤细胞株的影响及胶质瘤细胞MGR2放射抗拒性的诱导After11months，thesublinewasnamedMGR2R(MGR2radiationinduction)．DoublingtimesandgrowthcurvesofMGR2，T98GandtheirsurvivalcellsfollowingirradiationweredeterminedbyMTTassay．Dose—survivalcurves，radiobiologicalparametersandthesurvivalfractionat2Gy(SF2)w

2、eredeterminedbyusingcolony-formingassayandline-quadraticmodel．Thevariationoftheircellcycleswasinvestigatedbyusingflowcytometryandcellcyclesynchronizationofserum-starvation．Severalgeneexpressionassociatedwithradiosensitivityweredeterminedbywesternblotanalysisinthesecells．Activityo

3、fDNA--dependentproteinkinase(DNA·-PK)wasdeterminedbyDNA—PKassaysystemofSigmaTechnology．Results：TherewasnomorphologicalchangeofMGR2，T98GandthesurvivalcellsfollowingirradiationbyX-rayunderlightmicroscope．DoublingtimeofMGR2was3．6days，whileM-2(MGR2following2Gyirradiation)，M-2-2(MGR2f

4、ollowing2Gyirradiation2times)，M-5(MGR2following5Gyirradiation)were4．3days，4．8daysrespectively．DoublingtimesofT98GandT-3，T-3·3，andT-5(T98GfollowingX-rayirradiationat3Gy,3Gy2timesand5Gy,respectively)were2．3days，3．6days，4．0days，and4．6days，respectively．SF2ofM，M·2，M-2-2，M-5wereO．208，0

5、．228，0．252，0．23，andofT，T-3，T-3．3’T-5were0．453，0．464，O．494，and0．533，respectively．WhileSF2ofMGR2Rwas0．478andhigherthanthatofMGR2(卢-6。062，P=O．040)．ThedistributionofG1phasebyserum—starvationsynchronizationofMGR2，M-2，M一2-2，M-5were54．8％，53．7％，57．7％，64．7％，andthoseofT98G,T-3，"1"-3—3，T．5w

6、ere85．4％，62．4％，72．2％，and64．4％．respectively．Therewasgreatvariationindistributionofcellcyclebeforesynchronizationandthatofsynchronizationafter36hwithoutserumculture．HalfofcellnumberinvestigatedbyflowcytometryinMGR2，M·2，M-2-2，M-5，T98G,T-3，T_3-3，T-5andMGR2RwereblockedinGlphase，respec

7、tively．Twentyfourhou心afterlossofsynchronizationbyculturedwith10％serum，thedistributionofGlphaseinMGR2，M_2，M-2—2，M一5were35．9％，V《》。I．山大学x线对胶质瘤细胞株的影响及胶质瘤细胞MGR2放射抗拒性的诱导45．6％，57．1％，and53．7％，respectively．TherateofenteringnextphasefromGjphaseinsurvivalsublinecellsafterirradiationwasslowe

8、rthanthatofparentscells．While，inMGR2R，Gl