delta阿片受体激动剂对脓毒症大鼠肝线粒体呼吸功能的保护作用

《delta阿片受体激动剂对脓毒症大鼠肝线粒体呼吸功能的保护作用》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、万方数据浙江大学硕士学位论文英文摘要Protectiveeffectofdeltaopioidreceptoragoniston-o’‘●一●mJtochondrtalrespiratoryfunctioninaratmodelofseosisDepartmentofgeneralsurgery,ZhejiangUniversitySchoolofMedicineGraduateHuangSan,xiongSupervisorWuYu—lianProfessorAbstractobjective：TostudytheProtectiveeffectofdeltaopio

2、idreceptoragonistonmitochondrialrespiratoryfunctioninaratmodelofsepsis．Methods：。60SDratswererandomlydividedintoshamoperatedgroup(SO)，septicgroup(SEP)，andDADLEgroup(DADLE)．Sepsismodelwasproducedbycecalligationandpuncture(CLP)．InSOgroup，theabdomenwasopenedwithoutanyothertreatment．InDADLEgr

3、oup，DADLE(0．5mg／m1)wasadministeratadoseof5ml／kgbyintra—p．eritoneali巧ectionafterCLP．Allratsweresacrificed8hoursafterCLRAIT，AST,TBILinbloodweredetermined．MitochondrialrespiratoryfunctionWasevaluatedbytheclarkoxygeneletrode．ThelevelsofATP，ADP,AMPweredeterminedbyhighperformanceliquidchromato

4、graphy(HPLC)．Specimensoflivertissueweretakenforhistopathologicalanalysis．Results：RCRandADP／OinDADLEgroupweremuchhigherthaninSEPgroup俾<0：05)．ATPinDADLEgroupsweremuchhigherthanSEPgroup俨<0．05)，whileALTandASTofDADLEgroupweresignificantlylowerthanSEPgroup(P<口酊)．IV万方数据浙江大学硕士学位论文英文摘要Histopathol

5、ogicchangesunderlightmicroscopeoflivertissuewerelighterinDADLEgroup．Conclusion：LiverandmitochondrialinjuryatearlystageofSEPSIScharacterizedbyDeteriorationofmitochondrialrespiratoryfunction，cellintegrityandenergymetabolismwerereversible．DADLEprotectedmitochondrialrespiratoryfunctionandimp

6、rovedthehepatocellularenergymetabolisminsepsis．KeyWords：DADLE；Sepsis；CLP；MitochondriaV万方数据浙江大学硕士学位论文英文缩略词表VI万方数据目录致{射⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．I中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯一II英文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IV英文缩略词表⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯VI录正文1．绪论⋯⋯⋯⋯⋯⋯⋯⋯⋯

7、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．12．材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．43．结月之⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．94．讨论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯145．结论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯186实验的局限性及今后研究方向⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。⋯⋯⋯⋯⋯一19参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯