ctgfsirna 改善stz诱导糖尿病大鼠视网膜细胞凋亡

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1、CTGFsiRNA¸ÄÉÆSTZÓÕµ¼ÌÇÄò²¡´óÊóÊÓÍøĤϸ°ûµòÍö×÷ÕߣºÑîºê࣬³ÂÏþ¡£¬ÁõÕÜÀö£¬Áõ½à£¬²·Á¢Ãô¡¾ÕªÒª¡¿¡¡¡¡Ä¿µÄ£ºÌ½ÌÖCTGF¶ÔÌÇÄò²¡´óÊóÊÓÍøĤÄÚϸ°ûµòÍöµÄÓ°Ïì¡£·½·¨£º½«60´óÊó·ÖΪ¶ÔÕÕ×飬ÌÇÄò²¡4£¬8£¬12£¬16wk×éºÍ¸ÉÔ¤×é¡£ÌÇÄò²¡´óÊóÓ¦ÓÃSTZ¸¹Ç»×¢ÉäÓÕµ¼³ÉÄ£¡£¸ÉÔ¤×é´óÊóÓÚÌÇÄò²¡³ÉÄ£

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3、ýΪ25.8¸ö/mm2¡£CTGF±í´ïÓÚ8wkʱ³öÏÖÉý¸ß£¬µ½16wkʱÉý¸ß¸üÃ÷ÏÔ¡£CTGFsiRNA´¦ÀíºóµòÍöϸ°ûÊýÃ÷ÏÔ½µµÍ¡£ÊÓÍøĤÄÚCTGFºÍϸ°ûµòÍöÖ®¼ä¾ßÓÐÃ÷ÏÔµÄÏà¹ØÐÔ¡£½áÂÛ£ºCTGF²ÎÓëÁËÌÇÄò²¡ÊÓÍøĤÔçÆÚ²¡±äµÄϸ°ûµòÍö»úÖÆ£¬CTGFsiRNAÓÐÀûÓÚ¸ÄÉÆÌÇÄò²¡ÔçÆÚÊÓÍøĤÓÉÓÚµòÍöËùÖµÄϸ°û¶ªÊ§¡£¡¾¹Ø¼ü´Ê¡¿µòÍ

4、ö;CTGF;ÊÓÍøĤ;ÌÇÄò²¡25¡¡¡¡Abstract¤rAIM:TodetecttheeffectofCTGFontheapoptosisinthediabeticretinawithsmallinterferingRNAs(siRNA)targetingwithCTGF.¤rMETHODS:Atotalof60ratsweredividedintosixgroupsincludingcontrolgroup,diabetic4,8,12,16weeksgroup,andinterferencegroup

5、.DiabeticratswereinducedbySTZintraª²peritoneal.At4,8,12,16weeksafterdiabeticsettingup,retinaswereobtainedfromcontrol,diabeticratsanddiabeticanimalstreatedbyintravitrealinjectionofCTGFsiRNAtosuppresstheexpressionofCTGFmRNA.RetinalcellsapoptosiswasdetectedbyTunnels

6、tainingandmRNAexpressionofCTGFwasanalyzedbyRTª²PCR.RESULTS:ThelevelsofCTGFandtheapoptosisintheretinasofdiabeticratsweresignificantlyhigherthanthoseinthecontrols.Apoptosisoccurredat4weeksafteradiabeticmodelsettingup,becameseriouswiththediabetesdeveloping,whileCTGF

7、elevatedat8weeks.Thecellapoptosiscountsincreasedto25.8cells/mm2at24weeksofdiabetes.SiRNAª²mediatedinhibitionofCTGFmRNAresultedinasignificantdecreaseinapoptosis.SignificantcorrelationswerefoundbetweenCTGFandapoptosisintheretina.¤rCONCLUSION:Theseresultssuggestthat

8、CTGFmightbeinvolvedinretinalcellsapoptosiswhichisacharacteristic25ofearlydiabeticretina.siRNAtargetingCTGFseemstohavetheadvantageofamelioratingretinalcellslost