白黎芦醇(resveratrol)对食管癌ec109细胞生物学作用的初步研究

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1、汕头大学医学院硕士学位论文mol/Ltreatmentgroups.TheresultsofDNAagarosegelelectrophoresisshowedthatDNAladderappearedobviouslyat500μmol/LRes-treatedgroups.TheultrastructuralchangesofEC109cellstreatedwithresveratrolwereasfollowing:thenumberofmicrovillusonthesurfaceofplasmamembranedecreasedandcavitati

2、onscanbeobservedandchromatinmarginationwasfrequentinnucleolus.ResveratrolaffectedEC109cellcycledistributionandarrestedEC109cellinS-phaseaccompaniedwithreducingG0/G1phasecells.Sub-G1peakappearedobviouslyaftertreatmentwith125、250、500µmol/Lresveratrolwhendetectedbyflowcytometry.Theratio

3、ofAnnexinⅠ/β-actinwere0.1514、0.2019、1.2877、1.3263、1.8854、1.9585、2.457,respectively,whentreatedwithresveratrolfor0，1，3，6，12，24and48hmeasuredbywesternblot.ResveratrolenchancedtheexpressionofAnnexinⅠintime-dependentmanner.【Conclusions】ResveratrolcaninhibitthegrowthandproliferationofEC10

4、9cellsintime-anddose-dependentmanner.ResveratrolcaninducethecellcyclearrestintheSphaseofEC109cells,inhibitDNAsynthesisandinducecellapoptosisindose-dependentmanner.Resveratrolcanup-regulatetheexpressionofAnnexinⅠintime-dependentmanner.【KeyWords】resveratrol;esophagealcarcinomacells;gro

5、wthinhibition;apoptosis;AnnexinA1IV汕头大学医学院硕士学位论文英文缩略词英文缩写英文全称中文名称ResResveratrol白藜芦醇DMSOdimethylsulphoxide二甲基亚砜DTTdithiothreitol二硫苏糖醇EDTAEthylenediaminetetraaceticacid乙二胺四乙酸MTT3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide四甲基偶氮唑盐APAmmoniumpersulfate过硫酸胺BSABovineserumalbu

6、min牛血清白蛋白hrhour小时kDkilodatton千道尔顿rpmrevolutionperminute转/分钟minminute分钟mlmilliliter毫升β-MEβ-mercaptoethanolβ-巯基乙醇ODopticaldensity光密度EBethidiumbromide溴化乙锭PAGEPolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳PBSphosphatebufferedsaline磷酸盐缓冲液PIpropidiumiodide碘化吡啶Aprotinin抑蛋白酶肽Leupeptin亮抑蛋白酶肽PMSFph

7、enylmethylsulfonylfluoride苯甲磺酰氟SDSSodiumdodecylsulfate十二烷基硫酸钠TBSTris-bufferedsalineTris缓冲盐溶液TEMEDN,N,N,N,-tetramethyl-ethylerediamine四甲基乙烯二胺TrisN-trishydroxymethylaminomethaneN-三羟甲基氨基甲烷Acracrylamide丙烯酰胺V汕头大学医学院硕士学位论文目录中文摘要•••••••••••••••••••••••••••••••••••••••••••••••••••••••Ⅰ英文摘要••

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