molecular characterization of mulberry

《molecular characterization of mulberry》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、ResearchArticleReceived:5February2011Revised:16August2011Accepted:11October2011PublishedonlineinWileyOnlineLibrary:20December2011(wileyonlinelibrary.com)DOI10.1002/jsfa.4749Molecularcharacterizationofmulberry(Morusspp.)genotypesviaRAPDandISSRMuzaffer˙Ipek,a∗LutfiPirlak¨aandSalihKafkas

2、bAbstractBACKGROUND:Inrecentyears,DNA-basedmarkershavebeenusedquiteextensivelybecauseoftheirmanyadvantagesoverthetraditionalmorphologicalandbiochemicalmarkers.Manystudieshaveshownthatmolecularmarkersareusefulindelineatingthegeneticrelationshipsamongcloselyrelatedmulberrygenotypesandc

3、ultivars.Thus,inthepresentstudy,polymerchainreactionbasedDNAfingerprintingtechniqueswereusedtoinvestigatethegeneticrelationshipsamongmulberrygenotypesgrowingindifferentagro-climaticregionsofTurkey.RESULTS:20RAPDprimersgeneratedatotalof173bands,ofwhich157(90.75%)werepolymorphic.Asfor11

4、ISSRprimers,124bands(96.55%)werepolymorphicinatotalof128.ThesimilarityindexforRAPDtechniquerangedbetween0.240.98;25˙Is203with25˙Is112werefoundtobetheclosestgenotypes,while24Ke10and25˙Is123werethemostdistantones.AccordingtotheISSRresult,thegeneticsimilarityindexchangedbetween0.21095;2

5、5˙Is203with25˙Is112genotypesweretheclosest,while25˙Is08and01KaD2werethemostdistantones.CONCLUSION:TheRAPDandISSRmarkerswerefoundtobepromisingforassessinggeneticdiversityinmulberrygenotypes.
c2011SocietyofChemicalIndustryKeywords:RAPD;ISSR;mulberry;geneticsimilarityINTRODUCTIONcharact

6、ershavebeenpoorlyconducted.Ifhigh-yieldingandMulberriesbelongtothegenusMorusintheMoraceaefamily.qualityfruitphenotypeswereselectedandthenpropagated,About12mulberryspeciesarecultivatedworldwidefortheirmulberryproductioncouldbeincreased.foliage,fruit,timberorforornamentaluse.Amongthem,

7、MorusInrecentyears,DNA-basedmarkershavebeenusedquiteextensively5becauseoftheiradvantagesovertraditionalmorpho-alba,M.rubraandM.nigrahavebeenusedfortheirfruitsinbiochemicalmarkers.6,7MorphologicalmarkersarefewandmightsouthwestChinaintheFarEast(M.alba),inNorthAmerica(M.rubra)andinCentr

8、alAsia,Anato