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1、CharacterizationandexpressionofmicrobialinulinasegenesCriticalReviewsinMicrobiology,2013;39(2):152–165©2013InformaHealthcareUSA,Inc.G.-L.Liuetal.ISSN1040-841Xprint/ISSN1549-7828onlineDOI:10.3109/1040841X.2012.694411CriticalReviewsinMicrobiology2013RevIewARtIcle39Molecularch
2、aracterizationandexpressionofmicrobial2inulinasegenes152165Guang-LeiLiu,ZheChi,andZhen-MingChiFeb182012UnescoChineseCenterofMarineBiotechnology,OceanUniversityofChina,YushanRoad,Qingdao,ChinaMay132012AbstractManygenesencodingexo-andendo-inulinasesfrombacteria,yeastsandfilam
3、entousfungihavebeenclonedMay142012andcharacterized.Alltheinulinaseshaveseveralconservedmotifs,suchasWMND(E)PNGL,RDP,EC(V)P,SVEVF,QandFS(T),whichplayanimportantroleininulinasecatalysisandsubstratebinding.However,theexo-inulinases1040-841XproducedbyyeastshasnoconservedmotifSV
4、EVFandtheyeastsdonotproduceanyendo-inulinase.Exo-andendo-inulinasesfoundindifferentmicroorganismsclusterseparatelyatdistantpositionsfromeachother.MostoftheclonedinulinasegeneshavebeenexpressedinYarrowialipolytica,Saccharomycescerevisiae,Pichiapastoris,1549-7828Klyuveromyces
5、lactisandEscherichiacoli,respectively.Therecombinantinulinasesproducedandtheengineeredhostsusingtheclonedinulinasegeneshavemanypotentialapplications.Expressionofmostoftheinulinasegenes©2013InformaHealthcareUSA,Inc.isrepressedbyglucoseandfructoseandinducedbyinulinandsucrose.
6、However,thedetailedmechanismsoftherepressionandinductionarestillunknown.10.3109/1040841X.2012.694411Keywords:Inulinase,INU1gene,catalysismechanisms,evolution,repression,inductionIntroductionhydrolysiswithinulinasesoffersanalternativeapproachInnature,inulinisamixtureofpolysa
7、ccharidescom-toproducefructosefreeofundesirableby-products.posedof20–30fructoseunitchains[linkedbyβ-(2,1)-Inulinasesarefructofuranosylhydrolasesthatd-fructosyl-fructosebonds]ofvariouslengthswithatargetontheβ-2,1linkageofinulinandhydrolyzeitglucosemoleculeattheendofeachfruct
8、osechainandintofructoseandglucose.Accordingtotheiraction,producedbysomeplantssucha
