Characterization of a microbial consortium capable of degrading lignocellulose

《Characterization of a microbial consortium capable of degrading lignocellulose》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、BioresourceTechnology102(2011)9321–9324ContentslistsavailableatScienceDirectBioresourceTechnologyjournalhomepage:www.elsevier.com/locate/biortechShortCommunicationCharacterizationofamicrobialconsortiumcapableofdegradinglignocellulosea,⇑abaaaWeidongWang,LeiYan,ZongjunCui,YameiGao,

2、YanjieWang,RuiyongJingaCollegeofLifeScienceandTechnology,HeilongjiangBayiAgriculturalUniversity,Daqing163319,PRChinabCollegeofAgronomyandBiotechnology,ChinaAgriculturalUniversity,Beijing100193,PRChinaarticleinfoabstractArticlehistory:Amicrobialconsortium,designatedWCS-6,wasestabl

3、ishedbysuccessivesubcultivationinthepres-Received25February2011enceofricestrawunderstaticconditions.ThedegradationefficienciesofWSC-6for0.5gfilterpaper,Receivedinrevisedform13July2011cottonandricestrawafter3daysofcultivationwere99.0±0.7%,76.9±1.5%and81.3±0.8%,respec-Accepted19July2

4、011tivelyasdeterminedbygravimetricalmethods.NinebacterialisolateswereobtainedfromWCS-6pla-Availableonline24July2011tedunderaerobicconditions,andsequencingoftheir16SrDNAindicatedthatthesebacteriawererelatedtoBacillusthermoamylovoransBTa,PaenibacillusbarengoltziiSAFN-016,Proteobact

5、eriumS072,Keywords:PseudoxanthomonastaiwanensisCB-226,Rhizobiaceaestr.M100,Bacillussp.E53-10,BetaproteobacteriumLignocellulosedegradationHMD444,Petrobactersuccinimandens4BON,andTepidiphilusmargaritiferN2-214.DGGE(denaturinggra-MicrobialcommunityCulture-dependentdientgelelectropho

6、resis)andsequencingof16SrDNAsequencesamplifiedfromtotalconsortiumDNADGGErevealedthepresenceofsequencesrelatedtothoseofUreibacillusthermosphaericus,unculturedbacte-PhylogeneticanalysisriumcloneGC3,unculturedClostridiumsp.cloneA1-3,Clostridiumthermobutyricum,andClostridiumthermosucc

7、inogenesinadditiontothesequencesidentifiedfromtheculturedbacteria.Themicrobialcommunityidentifiedhereinisapotentialcandidateconsortiumforthedegradationofwastelignocel-lulosicbiomass.Ó2011ElsevierLtd.Allrightsreserved.1.Introduction2.MethodsLignocellulosicbiomass,whichconsistsmainly

8、ofcellulose(35–2.1.Cellulosicmaterials50