资源描述:
《Molecular diversity and differential expression of starch-synthesis genes in developing kernels of three maize inbreds》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、PlantCellRep(2009)28:1487–1495DOI10.1007/s00299-009-0748-5ORIGINALPAPERMoleculardiversityanddifferentialexpressionofstarch-synthesisgenesindevelopingkernelsofthreemaizeinbredsXiang-ZhenDingÆBei-GuoWangÆQing-HuaGaoÆQiongZhangÆGui-QinYanÆKeDuanÆJian-HuaHuangReceived:27April2009
2、/Revised:1July2009/Accepted:10July2009/Publishedonline:25July2009ÓSpringer-Verlag2009AbstractThemaizegenomeremainsabundantin(potentiallyisa3too).Themis-splicingeventsdirectlymoleculardiversity,andtherichgeneticdiversityofmaizeaccountedforwxlossoffunction.Contrarily,disruption
3、ofstarch-synthesisgenesiscrucialforcontrollingvarious50and30transcriptsequencemaycontributetotheabsencegraintraits.ToexploretheuniquemechanismcontrollingofGbssIIbandIsa2transcriptsinwaxyinbreds,respec-theadvantageouswaxytraitandcharacterizethemoleculartively.Besides,thesplici
4、ngofSugary1transcriptwasfeatureofgenesrelevanttostarchcompositionintwoelitedevelopmentallyregulatedinthenormalinbred,andDNAwaxyinbreds,expressionprofilingcombinedwithgenepolymorphismsweredetectedwithinSSIIIb-1geneinorganizationanalysiswasperformedinthemascomparedwaxyinbreds.to
5、onenormalinbred.Genotype-specificexpressionpat-ternswereobservedformostgenesstudied.ThewaxyKeywordsZeamaysStarch-synthesisgeneinbredswereshowntocontainmutationsinmultipleExpressionpatternNucleicpolymorphismstarch-synthesisgenes,namelygbssI(wx),gbssIIbandisa2Mis-splicingeve
6、ntMutationCommunicatedbyD.Somers.AbbreviationsWAPWeeksafterpollinationX.-Z.DingandB.-G.Wangcontributedequallytothiswork.GBSSGranule-boundstarchsynthaseSSSSolublestarchsynthaseElectronicsupplementarymaterialTheonlineversionofthisarticle(doi:10.1007/s00299-009-0748-5)containss
7、upplementarySBEStarchbranchingenzymematerial,whichisavailabletoauthorizedusers.DBEStarchdebranchingenzymeX.-Z.DingB.-G.WangQ.ZhangK.Duan(&)J.-H.Huang(&)ShanghaiKeyLaboratoryofAgriculturalGeneticsandBreeding,InstituteofBiologicalTechnique,ShanghaiAcademyofAgriculturalScien
8、ces(SAAS),IntroductionBeidiRoad2901,MinhangZone,201106Shanghai,China