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《Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments 》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、EnvironSciPollutRes(2015)22:3138–3148DOI10.1007/s11356-014-3574-4RESEARCHARTICLEChloroethenedegradationandexpressionofDehalococcoidesdehalogenasegenesinculturesoriginatingfromYangtzesedimentsIreneKranzioch&SelinaGanz&AndreasTiehmReceived:10June2014/Accepted:8September2014/Publishedonline:20Septembe
2、r2014#Springer-VerlagBerlinHeidelberg2014AbstractTheanaerobicDehalococcoidesspp.istheonlyIntroductionmicroorganismknowntocompletelydechlorinatethehazard-ouscompoundstetrachloroethene(PCE)ortrichloroetheneTheextensiveuseofchlorinatedethenesassolventsand(TCE)viadichloroethene(DCE)andvinylchloride(VC)
3、tosyntheticfeedstockshasresultedinwidespreadenvironmen-theterminalproduct,ethene.Inthisstudy,growthoftalcontamination.Theirstrongtoxicity,persistenceintheDehalococcoidesspp.(DHC)andtheexpressionofDHCenvironment,andcarcinogenicitymayposeseriousenviron-dehalogenasegenesweredemonstratedforYangtzeenric
4、h-mentalproblems.Groundwaterinthevicinityofindustrialmentcultures.Reductivedechlorinationofchloroethenesoc-sitesproducingorusingchlorinatedorganiccompoundsiscurredinYangtzesedimentwithouttheadditionofanyexter-oftencontaminatedwithchlorinatedethenes(Aktaşetal.nalauxiliarysubstrates.AllYangtzeenrichm
5、entcultures2012),andchlorinatedethenecontaminationhasbeenreport-completelydechlorinatedPCEandcis-DCEtoethene.Toedinsoilandgroundwaterworldwide,includinginGermany,investigateexpressionofthedehalogenasegenespceA,tceA,theUSA,Japan,andSouthKorea(SchmidtandTiehm2008;vcrA,andbvcA,aprotocolformessengerRNA
6、(mRNA)Carreón-Diazcontietal.2009;Iseetal.2011;Leeetal.2011).extractionfollowedbyreversetranscriptionandquantitativeInChina,theoccurrenceofchlorinatedethenesPCRanalysiswasestablished.Duringdechlorination,anin-(tetrachloroethene(PCE),trichloroethene(TCE),andvinylcreaseingenecopynumbersofpceA,tceA,and
7、vcrAwaschloride(VC))hasbeenreportedinsurfacewater(Lietal.observed.However,temporaryformationofmRNAwasonly2006;Hanetal.2011;Wolfetal.2013).InthelastfewmeasuredinthecaseofthedehalogenasegenestceAandvcrA.decad
