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isolation of dna-methyltransferase genes from strawberry

isolation of dna-methyltransferase genes from strawberry

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时间：2017-12-07

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1、PlantCellRep(2009)28:1373–1384DOI10.1007/s00299-009-0737-8ORIGINALPAPERIsolationofDNA-methyltransferasegenesfromstrawberry(Fragaria3ananassaDuch.)andtheirexpressioninrelationtomicropropagationLinlinChangÆZhihongZhangÆBaimingHanÆHeLiÆHongyanDaiÆPingHeÆHongzheTianReceived:18May2009/Accepted:14June2

2、009/Publishedonline:30June2009ÓSpringer-Verlag2009AbstractDNAmethylationcancontrolgeneexpressionThetranscriptionalexpressionsofbothFaMET1andandmayalsoplayaroleinplantdevelopment.MethylationFaDRMgenesweredownregulatedinmicropropagatedofcytosineresiduesinDNAisenzymaticallycatalyzedbyplants,andtheyw

3、ererecoveredinthefirstandsecondDNAmethyltransferases.Inthisstudy,full-lengthgenomicrunnergenerationsofmicropropagatedplants.However,genesandcDNAsofmethyltransferase(MET1)andtherewasaslighterdifferenceinglobalDNAmethylationdomain-rearrangedmethyltransferase(DRM)wereisolatedratesbetweenmicropropagat

4、edplantsandconventionallyfromstrawberry(Fragaria9ananassaDuch.).Twopropagatedplants.Therefore,therewasnopositiverelationgenomicclones(FaMET1aandFaMET1b)encodingMET1betweenglobalDNAmethylationratesandtheexpressionhadopen-readingframeof4,695and4,671nucleotideswithlevelsofMET1andDRMgenes.twointrons,

5、respectively.Aminoacidsequencecompari-sonindicatedhighsimilarity(98.72%identity)ofstraw-KeywordsMETDRMReal-timePCRberryMET1proteintootherplantMET1sequences.TheFragaria9ananassaMicropropagationfull-lengthcDNAofstrawberryDRMgenes(FaDRMa,FaDRMbandFaDRMc)were2,273,2,282and2,288bp,Abbreviationsres

6、pectively.TenintronswithdifferentsizesweredispersedIBAIndole-3-butyricacidinFaDRMgenes.Similarly,FaDRMa,FaDRMbandMETMethyltransferaseFaDRMchadhigh-sequencesimilarityoverall.ExpressionsDRMDomain-rearrangedmethyltransferaseofstrawberryMET1andDRMgeneswerecomparedamongDMTDNA-(cytosine-5)methyltransfe

7、raseinvitro-micropropagatedplants,generationsofmicro-HPLCHigh-performanceliquidchromatographypropagatedplantsandconventionallypropagatedplants.CommunicatedbyR.Reski.IntroductionElectronicsupplementarymaterialTheonlinev

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