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1、BMCPlantBiologyBioMedCentralResearcharticleOpenAccessExploringvalidreferencegenesforgeneexpressionstudiesinBrachypodiumdistachyonbyreal-timePCRShin-YoungHong†1,PilJoonSeo†1,Moon-SikYang2,FengningXiang3andChung-MoPark*1Address:1DepartmentofChemistry,SeoulNationalUniversity,Seoul,15
2、1-742,Korea,2DivisionofBiologicalSciencesandtheResearchInstituteforBioactiveMaterials,ChonbukNationalUniversity,Jeonju,561-756,Koreaand3SchoolofLifeSciences,ShandongUniversity,Jinan250100,Shandong,PRChinaEmail:Shin-YoungHong-cutesy77@yahoo.co.kr;PilJoonSeo-dualnt83@snu.ac.kr;Moon-
3、SikYang-mskyang@chonbuk.ac.kr;FengningXiang-xfn0990@sdu.edu.cn;Chung-MoPark*-cmpark@snu.ac.kr*Correspondingauthor†EqualcontributorsPublished:7November2008Received:29May2008Accepted:7November2008BMCPlantBiology2008,8:112doi:10.1186/1471-2229-8-112Thisarticleisavailablefrom:http://w
4、ww.biomedcentral.com/1471-2229/8/112©2008Hongetal;licenseeBioMedCentralLtd.ThisisanOpenAccessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense(http://creativecommons.org/licenses/by/2.0),whichpermitsunrestricteduse,distribution,andreproductioninanymedium,provid
5、edtheoriginalworkisproperlycited.AbstractBackground:ThewildgrassspeciesBrachypodiumdistachyon(Brachypodiumhereafter)isemergingasanewmodelsystemforgrasscropgenomicsresearchandbiofuelgrassbiology.Adraftnucleargenomesequenceisexpectedtobepubliclyavailableinthenearfuture;anexplosionof
6、geneexpressionstudieswillundoubtedlyfollow.Therefore,stablereferencegenesarenecessarytonormalizethegeneexpressiondata.Results:AsystematicexplorationofsuitablereferencegenesinBrachypodiumispresentedhere.Ninereferencegenecandidateswerechosen,andtheirgenesequenceswereobtainedfromtheB
7、rachypodiumexpressedsequencetag(EST)databases.Theirexpressionlevelswereexaminedbyquantitativereal-timePCR(qRT-PCR)using21differentBrachypodiumplantsamples,includingthosefromdifferentplanttissuesandgrownundervariousgrowthconditions.Effectsofplantgrowthhormoneswerealsovisualizedinth
8、eassays.Theexpressionstabilityoft
