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《Effects of drought on gene expression in maize reproductive and leaf meristem tissue revealed by RNA-Seq》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、EffectsofDroughtonGeneExpressioninMaizeReproductiveandLeafMeristemTissueRevealedbyRNA-Seq1[W][OA]22,345AkshayKakumanu,MadanaM.R.Ambavaram,CurtisKlumas,ArjunKrishnan,UtlwangBatlang,ElijahMyers,RuthGrene*,andAndyPereiraVirginiaBioinformaticsInstitute(A.Ka.,M.M.R.A.,A.Kr.,U.B.,A.P.),Departmen
2、tofPlantPathology(A.Ka.,R.G.),andGenetics,Bioinformatics,andComputationalBiologyProgram(C.K.,E.M.),VirginiaTech,Blacksburg,Virginia24061;andCrop,Soil,andEnvironmentalSciences,UniversityofArkansas,Fayetteville,Arkansas72701(A.P.)Droughtstressaffectscerealsespeciallyduringthereproductivestag
3、e.Themaize(Zeamays)droughttranscriptomewasstudiedusingRNA-Seqanalysistocomparedrought-treatedandwell-wateredfertilizedovaryandbasalleafmeristemtissue.Moredrought-responsivegenesrespondedintheovarycomparedwiththeleafmeristem.GeneOntologyenrichmentanalysisrevealedamassivedecreaseintranscript
4、abundanceofcelldivisionandcellcyclegenesinthedrought-stressedovaryonly.AmongGeneOntologycategoriesrelatedtocarbohydratemetabolism,changesinstarchandSucmetabolism-relatedgenesoccurredintheovary,consistentwithadecreaseinstarchlevels,andinSuctransporterfunction,withnocomparablechangesoccurrin
5、gintheleafmeristem.Abscisicacid(ABA)-relatedprocessesrespondedpositively,butonlyintheovaries.Relatedresponsessuggestedtheoperationoflowglucosesensingindrought-stressedovaries.Thedataarediscussedinthecontextofthesusceptibilityofmaizekerneltodroughtstressleadingtoembryoabortionandtherelative
6、robustnessofdividingvegetativetissuetakenatthesametimefromthesameplantsubjectedtothesameconditions.OurworkinghypothesisinvolvessignalingeventsassociatedwithincreasedABAlevels,decreasedglucoselevels,disruptionofABA/sugarsignaling,activationofprogrammedcelldeath/senescencethroughrepressionof
7、aphospholipaseC-mediatedsignalingpathway,andarrestofthecellcycleinthestressedovaryat1dafterpollination.Increasedinvertaselevelsinthestressedleafmeristem,ontheotherhand,resultedinthattissuemaintaininghexoselevelsatan“unstressed”level,andatlowerABAlevels,whichwa
