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1、VirusResearchELSEVIERVirusResearch35(1995)277-289MolecularcharacterizationoftheSproteinsoftwoenterotropicmurinecoronavirusstrainsSatoshiKunitaa,1LinongZhangb,FelixR.Hornbergerb,SusanR.Comptona,.aSectionofComparatit:eMedicine,YaleUniL,ersitySchoolofMedicine,P.O.Box208016,New
2、Haven,CT06520-8016,USAbInstituteofLaboratoryAnimalScience,Ung,ersityofZurich,Zurich,SwitzerlandReceived22July1994;revised15September1994;accepted15September1994AbstractEnterotropicstrainsofroutinecoronaviruses(MHV-YandMHV-RI)differextensivelyintheirpathogenesisfromtheprotot
3、ypicrespiratorystrainsofmurinecoronaviruses.Inanefforttodeterminewhichviralproteinsmightbedeterminantsofenterotropism,im-munoblotsofMHV-YandMHV-RIvirionsusinganti-S,-Nand-Mprotein-specificantiserawereperformed.TheuncleavedMHV-YandMHV-RISproteinsmigratedslightlyfasterthanthe
4、MHV-A59Sprotein.TheMHV-YSproteinwasinefficientlycleaved.TheMHV-Y,MHV-RIandMHV-A59NandMproteinsshowedonlyminordifferencesintheirmigration.TheSgenesofMHV-YandMHV-RIwerecloned,sequencedandfoundtoencode1361and1376aminoacidlongproteins,respectively.Thepresenceofseveralaminoacids
5、changesupstreamfromthepredictedcleavagesiteoftheMHV-YSproteinmaycontributeitsinefficientcleavage.AhighdegreeofhomologywasfoundbetweentheMHV-RIandMHV-4Sproteins,whereasthehomologybetweentheMHV-YSproteinandtheSproteinsofotherMHVstrainswasmuchlower.Theseresultsindicatethatthee
6、nterotropismofMHV-RIandMHV-YmaybedeterminedbydifferentaminoacidchangesintheSproteinand/orbychangesinotherviralproteins.Keywords:Mousehepatitisvirus;Coronavirus;Sglycoprotein*Correspondingauthor.Fax:+1(203)7857499.Currentaddress:InstituteofLaboratoryAnimalResearch,SchoolofSc
7、ience,KitasatoUniversity,Kanagawa,Japan,0168-1702/95/$09.50©1995ElsevierScienceB.V.AllrightsreservedSSDI0168-1702(94)00089-1278S.Kunitaetal./VirusResearch35(1995)277-289I.IntroductionMousehepatitisvirus(MHV),asingularnameforseveralmurinecoronaviruses,causesawidespectrumofdi
8、seasesrangingfrommildenteritisorrhinitistofatalhepatitisorencephalitis.MHVstrainsc
