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1、ArchVirol(2000)145:1133–1147Molecularcharacterizationandpathogenesisoftransmissiblegastroenteritiscoronavirus(TGEV)andporcinerespiratorycoronavirus(PRCV)fieldisolatesco-circulatinginaswineherdL.Kim,J.Hayes,P.Lewis,A.V.Parwani,K.O.Chang,andL.J.SaifFoodAni
2、malHealthResearchProgram,DepartmentofVeterinaryPreventiveMedicine,OhioAgriculturalResearchandDevelopmentCenter,TheOhioStateUniversity,Wooster,Ohio,U.S.A.AcceptedDecember19,1999Summary.TGEVreplicatesinintestinalenterocytesandcausesdiarrheainyoungpigs.PRC
3、V,aspike(S)genedeletionmutantofTGEVwithanalteredrespiratorytissuetropism,causesmildorsubclinicalrespiratoryinfections.ComparisonsofTGEVandPRCVstrainssuggestthattropismandpathogenicityareinfluencedbytheSgeneandORF3,respectively.Recently,outbreaksofTGEofre
4、ducedvirulencewerereportedinthefield.WeinvestigatedasimilarsuspectTGEVoutbreakofreducedvirulenceinnurserypigsfromaswineherdintheMidwest.ATGEVstrain(BW021898B)wasisolatedinswinetesticularcellsfromgutcontentsofadiarrheicpigandthreePRCVstrains(BW126,BW154,B
5、W155)wereisolatedfromnasalswabsfromnormalTGEV-seronegativesentinelpigsincontactwiththediarrheicpigs.SequenceanalysisoftheTGEVisolateinthepartialSgeneandORF3/3aandORF3-1/3brevealedhighhomologywithen-teropathogenicTGEVstrains.Gnotobioticpiginoculationandh
6、istopathologicalresultsrevealedthatthisTGEVisolateretainedvirulenceeventhoughinthefieldoutbreakthediarrhealdiseasewasofreducedseverity.SequenceanalysisoftheSgenedeletionregionofthethreePRCVisolatesrevealedidenticaldeletionsbe-tweennt105–752,whichdifferfr
7、omdeletionspreviouslyreportedamongPRCVstrains.ThethreePRCVisolateshadvariablesequencechangesinORF3/3aandORF3-1/3b,affectingtheORFsizeandaminoacidsequence.Thus,sequenceanalysisandpathogenicitystudiesindicatethatthisTGEVisolateresemblesotherenteropathogen
8、icTGEVstrains.Therefore,thereducedseverityofTGEobservedinthisherdmaybeduetotheongoingPRCVinfections,whichinduceantibodies1134L.Kimetal.cross-reactivewithTGEVandresultindecreaseddiseaseseverity.Theresultsoutlinedinthisstudyhighlig
