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1、Jou/TzalofGeneralVirology(1993),74,19811987.PrintedinGreatBritain1981MolecularcharacterizationoftheSproteingeneofhumancoronavirusOC43SamirMounirandPierreJ.Talbot*VirologyResearchCenter,InstitutArmand-Frappier,UniversitdduQudbec,531boulevarddesPrairies,La
2、val,Qudbec,CanadaH7N4Z3ThegeneencodingthespikeproteinoftheOC43strainproteolyticcleavagesite(RRSR)betweenaminoacidofhumancoronavirus(HCV-OC43)wasclonedandresidues758and759,yieldingS1and$2segmentsofsequenced.Thecompletenucleotidesequencerevealed84730and653
3、66Mr,respectively.Thepredictedaminoanopenreadingframeof4062nucleotidesencodingaacidsequenceofthespikeproteinofHCV-OC43hasproteinof1353aminoacidswithapredictedMrof91%identitywiththatoftheMebusstrainofbovine150078.Structuralfeaturesinclude22N-glycosylation
4、coronavirus,revealingmoresequencedivergenceinthesites,anN-terminalhydrophobicsignalsequenceof17putativebulbouspart(S1)thaninthepredictedstemaminoacids,anhydrophiliccysteine-richsequenceof35region($2).aminoacidsneartheCterminus,andapotentialHumancoronavir
5、uses(HCV)areenvelopedpositive-Yeageretal.,1992),possessesafusogenicactivity,isthestrandedRNAvirusesthatcauserespiratoryinfectionsmajortargetforantiviralneutralizingantibodies(Spaanandhavebeenassociatedwithgastrointestinalandetal.,1988;Daniel&Talbot,1990)
6、andcanalsobeneurologicaldisorders(Jouvenneetal.,1992;McIntosh,recognizedbyTlymphocytes(K6rneretal.,1991).1974;Macnaughton&Davies,1981;Murrayetal.,Duringmaturationandintracellulartransport,someS1992;Restaetal.,1985,Stewartetal.,1992;Talbot&moleculesarecle
7、avedbyhostcellproteasesprobablyJouvenne,1992;Tyrrell,1986).TheyarecategorizedintolocatedintheGolgiapparatustoyieldtwolargesubunitstwomajorantigenicgroups,representedbytheprototypecalledS1and$2(Franaetal.,1985).Primarysequencestrains229EandOC43(Macnaughto
8、netal.,1981;WegeanalysissuggestedthatthebulbouspartoftheSproteinetal.,1982).isformedbytheN-terminalhalfofthemolecule,S1TheHCV-OC43virioniscomposedoffourstructural(Cavanagh,1983;deGrootetal.,1987a).TheC-proteins.Threeofthem
