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1、ArchivesofVirologyArchivesofVirology86,101--t08(1985)©bySpringer-Vertag1985OligonueleotideFingerprintsofAntigenieallyRelatedBovineCoronavirusandHumanCoronavirus0C43ByW.LAPPsandD.A.B~A~Depart~mentofMicrobiology,TheUniversityofTennessee,Knoxville,Tennessee,U.S.A.With2FiguresAccept~edDecemb
2、er12,1984SummaryVirion]%NAsfromthebovineentericcoronavirusandthehumanrespiratorycoronavirus0C43werecomparedbyonedimensionalgelelectro-phoresisandbyoligonueleotidefingerprinting.Foreachvirus,approxi-mately55percentoftheRNAmigratedasa6.8Mdspecies,t0percentasa0.68Mdspecies,and15percentashet
3、erogeneoussmallmolecularweightI~NA.Asequencehomologyofgreaterthan96percentwasobservedbetweenthe6.8Mdspeciesfromthetwoviruses.The0.68MdRNAisapparentlyanintravirion,subgenomic,polyadenylatedmoleculebasedonRNAsestudies,oligo(dT)-cellulosechromatography,andhybridizationtoaeDNAcloneofthe3'ter
4、minal1.19Kbregionofthebovinecoronavirusgenome.IntroductionThebovineentericcoronavirus(BCV)causesasevereenteritisinyoungcalves(18).Thehumanrespiratorycoronavirus0C43(HCV0C43)causesonlyamildupperrespiratorydiseaseinhumansofallages(16).Recentevidencesuggeststhatthesevirusesarestructurallycl
5、oselyrelated.Bothagglutinateerythrocytesfrommice,rats,andchickens(10,15,19),andbothshareantigensasdeterminedbyimmunofluorescence(20).Nobiologicalorstructuraldataexiststorigorouslydifferentiatebetweentheseviruses.Becauseasignificantfractionofthehumanpopulation(7to69percent)bothinEuropeand
6、theUnitedStatescarryantibodiesthatareabletoneutralize,immunoprecipitate,andindirectlyimmunofluorescethebovinecoronavirus(22),thepossibilityexiststhattilebovinecorona~drust02W.LA~2PSandD.A.B~tA~:andthehumancoronavirus0C43aretwonamesforthesameviruswithzoonoticpotential.Inthisreportwedescri
7、bestudiesdesignedtocomparegenomesequencesbetweenthetwoviruses.Thebovinecoronavirus(Mebusstrain)andthehumanrespiratoryeoronavirus0C43,whengrownonthesamehumancellline,shareasequencehomologyofgreaterthan96percentasdeterminedbyTIoligonucleotidefingerprintingbuteachpossessuniq
