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1、MedMicrobiollmmunolMedical(1981)169:169-178MicrobiologyandImmunology9Springer-Verlag1981ReactivityofAntibodiesinHumanSerumwithAntigensofanEnteropathogenicBovineCoronavirusJ.StorzIandR.Rott21DepartmentofMicrobiolog~ColoradoStateUniversity,FortCollins,CO80523,USA2InstitutforVirologic,Justus-Liebig-
2、Universit/itGiessen,Giessen,FederalRepublicofGermanyAbstract.Antibodiesinhumanserumagainstanenteropathogenicbovinecoronavirusweredetectedbydoubleimmunodiffusion(DID),neutralizationofinfectivity,indirectimmunofluorescence,andimmuneelectronmicroscopy.HumanserareactingintheDIDtestneutralizedtheinfect
3、ivityofthebovinecoronavirusestoindicesof2.5to~>5.Nineteenof40DID-negative,heat-inac-tivatedserahadneutralizingindicesof1to3.0.HumanserumwithneutralizingandDIDantibodiesproducedjuxtanuclearandcytoplasmicfluorescenceidenti-caltothatofbovineimmuneserumincellsinfectedwiththebovinecorona-virus.Antibodi
4、esinhumanandbovineserainteractedwiththepeplomeresofthebovinecoronavirus,mattingandbridgingthem,whenpresentinexcess,andfacilitatedformationoflargeviralaggregateswhenpresentinequivalentconcen-trations.Complementaddedtothevirus-antibodycomplexesdidnotalterspecificallythemorphologyofsingle,antibody-la
5、denviralparticlesorviralparticlesinaggregates.Evidenceofthetransmissionofcoronavirusfromex-perimentallyinoculatedcalvestoman,withensuinggastroenteritis,wasfoundbyelectronmicroscopictracingofthecoronavirusanditsvirus-antibodycom-plexes.IntroductionCoronavirusesareanimportantcauseofenteritisanddiarr
6、heainpiglets,calves,rodents,andotheranimalspecies[11].Intestinalinfectionsofmanwithcornaviruseswerede-monstratedrepeatedlybyelectronmicroscopy,butthesignificanceofthisinfectionasacauseofhumanentericdiseaseremainsunsettled[1,2,16].SharpeandMebus[17]detectedantibodiesneutralizinginfectivityofanenter
7、opathogenicbovinecoronavirusinseraofhumansubjects.Subsequently,antigenicrelationshipswerefoundbydirectandindirectimmunofluorescencebetweenthehumancoronavirusstrain0C43,anenteropathogenicbovinecoronavirus,thetype3
