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1、VIROLOGY183,442-445(1991)ComparisonofBovineCoronavirus(BCV)Antigens:MonoclonalAntibodiestotheSpikeGlycoproteinDistinguishbetweenVaccineandWild-TypeStrainsKHALIDA.HUSSAIN,JOHANNESSTORZ,ANDKONSTANTING.Kousoul~sDepartmentofVeterinaryMicrobiologyandParasitology,SchoolofVeterinaryMedicine,Lo
2、uisianaStateUniversity,BatonRouge,Louisiana70803ReceivedJanuary10,1991;acceptedMarch14,1991Monoclonalantibodies(MAbs)againsttwomajorstructuralproteinsofthecell-adaptedMebusstrainofbovinecoronavirus(BCV-L9)wereproducedandcharacterized.SevenMAbsreactedwiththepeplomericglycoprotein,gplOO/S
3、,whilethreeMAbsreactedwiththenucleoproteinp53/NinWesternblotanalysisofBCVpolypeptides.MAbstogplOO/SreactedwithdiscontinuousepitopesofgplOO/SinWesternsundermildbutnotunderstandarddenaturingconditions.Incontrast,MAbstop53/NreactedinbothtypesofWesterns,andthoseepitopeswerethuscontinuous.MA
4、bstop53/NfailedtoneutralizeBCVinfectivity,while4MAbstogplOO/SneutralizedBCVeffectively.CrossreactivityofMAbstogplOO/Sspecifiedbyfivevirulentwild-typestrainsandtwohighpassage,cell-culture-adaptedstrainsinmildlydenaturingWesternsandneutralizationassaysindicatedthattwoepitopeswereconserved
5、inallsevenstrains,whiletwoepitopesoftheavirulentstrainswerenotdetectedinthewild-typestrains.Non-neutralizingMAbsofgplOO/SreactedwithallsevenstrainsinWesternswiththeexceptionofoneMAbthatwasspecificforthehighlycell-adaptedstrainBCV-L9.o1991Academicpress.inc.Bovinecoronavirus(BCV)isamember
6、ofthecorona-ontheSandthreedomainsontheHEglycopro-viridaefamilythatcausesneonataldiarrhea,adiseasetein(13).withsubstantialeconomicimpactinthedairyandbeefAntigenicdiversityamongisolatesofseveralgroupscattleindustries.Theviralparticleisspherical,envel-ofcoronaviruseswasdocumented.MAbstoMHV
7、-4opedwithadiameterofaround100nm,possessingagpl80/90revealedlittleconservationamongisolatessingle-strandednonsegmentedRNAgenomeofposi-(14).ComparisonofTGEVstrainswithapanelofMAbstivepolarity.Theviralparticleiscomposedofsetsofevidencesdifferentialretentionofepitopes(10,11,15).fo
