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1、JournalofGeneralVirology(1990),71,487-492.PrintedinGreatBritain487NucleotidesequenceoftheglycoproteinSgeneofbovineentericcoronavirusandcomparisonwiththeSproteinsoftwomousehepatitisvirusstrainsPascalBoireau,1CatherineCruciereIandJaquesLaporte2.1LaboratoiredeVirologieMoldculaire,L.C.R.V.,C.N.E.V.A.,
2、22ruePierreCurie,BP67,94703Maison-AlfortCedexand2StationdeVirologieetd'ImmunologieMolbculaires,I.N.R.A.,C.R.J.J.,DomainedeVilvert,78350Jouy-en-Josas,FranceThegeneencodingthespikeglycoprotein(S)ofbovinetoS1(Mr104692)and$2(Mr84175)spikestructuralentericcoronavirus(BECV)wasclonedanditscom-proteins.Tw
3、oamphipathica-helices(aminoacids1007pletesequenceof4092nucleotideswasdetermined.to1077and1269to1294)whichmayconstitutetheThissequencecontainedasinglelongopenreading12nmstalkoftheviralspikewerealsoobserved;framewithacodingcapacityof1363aminoacids(Mranotheror-helix(aminoacids1305to1335)maybe150747).
4、Thepredictedproteinhad19N-glycosylationinvolvedintheanchorageofthespikeintheviralsites.Asignalsequencecomprising17aminoacidswasmembrane.Comparisonofthisproteinsequencetotheobservedstartingfromthefirstmethionineresidue.Adescribedhomologousmousehepatitis(MHV)strainpotentialpeptidasecleavagesitewaslo
5、catedbetweenA59andMHV-JHMSproteinsequencesledustoaminoacids763and767.ThesecleavagesexplainthesuggestthatMHV-A59andMHV-JHMSgenescouldmaturationoftheprimaryproductoftheSgenebederivedfromadeletionoftheBECVSgene.Bovineentericcoronavirus(BECV)wasfirstidentifiedBECVpossessesfivemainstructuralproteins,i.
6、e.abyelectronmicroscopyinfaecalsamplesofcalvesphosphorylatednucleocapsidprotein(N;50K),atrans-sufferingfromacuteenteritis(Mebusetal.,1973a).Themembranematrixglycoprotein(M;28K)andthreeinvolvementofBECVintheaetiologyofdiarrhoealpeplomerglycoproteins[S1,$2(S;spike)andhaemag-diseaseshasbeensuggestedi
7、nseveralstudies(Mebusetglutinin(HA)].GlycoproteinsS1(105K)and$2(95K)al.,1973b;Bridgeretal.,1978;Gouetetal.,1978).arethecleavageproductsoftheSgene-encodedprimaryDuringtheacutestageofinfection,virusparticlesareprod
