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1、JournalofGeneralVirology(1991),72,2817-2820.PrintedinGreatBritain2817Monoclonalantibodiesdifferentiatebetweenthehaemagglutinatingandthereceptor-destroyingactivitiesofbovinecoronavirusJ.Storz,1.G.Herrler,2D.R.Snodgrass,3K.A.Hussain,1X.M.Zhang,1M.A.Clark3andR.Ro
2、tt41DepartmentofVeterinaryMicrobiologyandParasitology,LouisianaStateUniversity,BatonRouge,Louisiana,U.S.A.,2InstitutfftrVirologie,Philipps-Universitiit,Marburg,Germany,3MoredunResearchInstitute,Edinburgh,U.K.and41nstitutfiirVirologie,Justus-Liebig-Universiti~t
3、,Giessen,GermanyArelativelysimpleandsensitivemethodisdescribeddirectedagainstthesurfaceglycoproteinsSandHEofwhichenablestheeffectofmonoclonalantibodiesBCV.Thereafter,thetestplateswereincubatedat(MAbs)onthereceptor-destroyingenzyme(RDE)and37°Ctoenabletheability
4、oftheMAbstopreventthehaemagglutination(HA)activityofbovinecorona-elutionofvirusfromBCV-erythrocytecomplexestovirus(BCV)tobeanalysedinoneassay.Alysateofbeassessed.NoinhibitionofRDEwasdetectablewithHRT-18cellsinfectedwiththeL9strainofBCVwasMAbsagainstglycoprotei
5、nS,whichhadHA1titresfoundtohaveahigherRDE:HAratiothanpurifiedrangingfrom1:16to1:128.Ontheotherhand,MAbsvirus.At4°CthelysateinducedanHApatternwhichdirectedagainstglycoproteinHEhadsimilarHA1completelydisappeareduponraisingofthetempera-titres,buttheyinhibitedelut
6、ionof8HAunitsofBCVtureto37°C.ThisL9-infectedcelllysatewasusedtoattitresofupto1:65000.determinetheHAinhibition(HAI)titresofMAbsBovinecoronavirus(BCV)isanenteropathogenwhichsequenceoftheSgeneofBCVencodes1363aminocausesseverediarrhoeainneonatalcalves(Mebusetal.,a
7、cids,withanN-terminalsignalsequenceanda1973)andwhichisimplicatedaetiologicallyinwintertransmembranesequenceneartheC-terminalend.dysenteryofadultcattle(Saifetal.,1988).BCVCleavageoftheSglycoproteinintoproteinsS1and$2isrepresentsoneofthebettercharacterizedhaemag
8、-predictedtooccuratanRRSRRorRRSVRsequenceatglutinatingcoronaviruses.Fourmajorstructuralproteinspositions764to768(Zhangetal.,1991;Parkeretal.,areassociatedwithinfectiousBCV(King&Bri
