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1、VeterinaryMicrobiology,20(1989)111-122111ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsCharacterizationofMonoclonalandPolyclonalAntibodiestoBovineEntericCoronavirus:EstablishmentofanEffecientELISAforAntigenDetectioninFecesC.-P.CZERNYandW.EICHHORNW.H.O.CollaboratingCen
2、treforCollectionandEvaluationofDataonComparativeVirology,InstituteofMedicalMicrobiology,InfectiousandEpidemicDiseases,VeterinaryFaculty,Ludwig-MaximiliansUniversity,VeteriniirstrasseI3,8000Munich22(F.R.G.)(Acceptedforpublication16December1988)ABSTRACTCzerny,C.-P.andEichhorn,W,,1989.Cha
3、racterizationofmonoclonalandpolyclonalantibodiestobovineentericcoronavirus:establishmentofanefficientELISAforantigendetectioninfeces.Vet.Microbiol.20:111-122.Monoclonalantibodiestobovineentericcoronavirus(BEC)wereproduced.Additionally,polyclonalantibodiesweremadeinrabbitsandguineapigsa
4、ndextractedfromtheyolkofim-munizedhens.Theantibodieswerecharacterizedbyneutralizationtest,hemagglutinationinhi-bitiontest,enzyme-linkedimmunosorbentassay(ELISA)andimmunoblotting.Neutralizingan-tibodytitersofpolyclonalantiserarangedfrom1:1280to1:40000.Onlyoneoutof908hybridomacoloniestes
5、tedsecretedantibodieswithneutralizingactivity.ByELISA,polyclonalseraexhibitedhighbackgroundreactionsthatcouldbesignificantlyreducedbytreatmentwithkaolininthecaseofrabbitsera.AttemptstoestablishanELISAforBECantigendetectionbasedonpolyclonalserafailedduetolowsensitivityandspecificity.Opt
6、imalresultswereachievedwhenamixtureoftwomonoclonalantibodieswascoatedontomicroplatesforantigencapture,whilerabbithyperimmuneserumservedasdetectingantibodiesinanindirectassay,Thecombinationofthetwomonoclonalantibodiesdidnotincreasesensitivitysynergistically,butinacompensatoryfashion,pro
7、bablybecauseofepitopedifferencesbetweenBECfieldstrains.INTRODUCTIONBovineentericcoronaviruses(BEC)areimportantpathogensinducingdiarrheainnewborncalvesworldwide.Theyarefoundaloneorinassociationwithrotaviruses,enterotoxigenicEscherichiacoliorcryptosporidia(BaljerandBachmann,1980;Reynol
