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1、JVetDiagnInvest7:538-539(1995)BRIEFCOMMUNICATIONSPlaquevariationsinclinicalisolatesofbovinecoronavirusSanjayKapil,CindyChard-Bergstrom,PamBolin,DeniseLandersBovinecoronavirus(BCV)causesenterocolitisandrespi-hyde,100%acetone,and10%neutralbufferedformalinas4ratorydiseaseinyoungcalvesandmaycausechroni
2、cdi-fixativesandfoundthatbufferedformalingavethebestre-1arrhea(winterdysentery)inadultcattle.Tounderstandthesults.After1hour,theagarosewasremoved.Platesweremolecularbasisoftissuetropismandvirulence,andtostudywashedoncewithphosphate-bufferedsalinetoremovere-biologicaldifferencesbetweenclinicalisolat
3、es,itisimportantsidualformalin.Fluorescein-labeledantibovinecoronavirusftoplaquepurifyBCV.Plaqueassaycanalsobeusedtostudyconjugatewasaddedandplateswereincubatedforanhourtheeffect(s)ofantiviraldrugs,suchashygromycinB,onBCVat37C.Theplateswerewashed3timeswithPBS,coverslipsreplicationinvitro.Moreover,t
4、hereisanurgentneedtowereaddedandplateswereexaminedbyfluorescencemi-selectbettervaccinecandidatesanddevelopaneffectivecroscopy.modifiedlivevirusvaccineagainstBCV,andcandidatevac-Theplaquesizesandtheviraltiters(plaque-formingunitscineisolatesmustbeplaquepurifiedforcharacterization.[PFU]/ml)areshownin
5、Table1.HRT-18cellsallowedef-56BecauseisolatesofBCVareobtainedfromfecalsamplesthatficientreplicationofBCV(10-10PFU/mlatpassage4ofcontainawidevarietyofbacteriaandviruses,plaquepuri-theviruses)butHRT-18cellsdonotallowreplicationofallficationisessential.Thispaperdescribesthedevelopmentthefieldisolateso
6、fBCV(KapilS,DonnellyC:1994,AbstofaplaqueassaytostudyplaquevariationinrecentisolatesProc75thConfResWorkersAnimDis,P77).AmongtheofBCV(1993-1994)andtostudytheantiviraleffectsofeightclinicalisolatesofBCV,onlyonewasnoncytopathichygromycinBonBCVinvitro.(WI-9)anddidnotproduceplaques.However,thepresenceHum
7、anrectaltumor-18(HRT-18)cellswereplatedinofbovinecoronaviralantigenwasconfirmedbyafluorescenta6-mmtissueculturedishesandgrowninminimumessentialantibodytest.Onecytopathicisolate(WI-10)producedmedium(MEM)with
