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1、VeterinaryImmunologyandImmunopathology,17(1987)425-439425ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsDEVELOPMENTOFNASAL,FECALANDSERUMISOTYPE-SPECIFICANTIBDDIESINCALVESCHALLENGEDWITHBOVINECORONAVIRUSORROTAVIRUSL.J.SAIFFoodAnimalHealthResearchProgram,OhioAgriculturalResearchandD
2、evelopmentCentre,TheOhioStateUniversity,Wooster,Ohio44691,USAABSTRACTSaif,L.J.,1987.Developmentofnasal,fecalandserumisotype-specificantibodiesincalveschallengedwithbovinecoronavirusorrotavirus.Vet.Immunol.Immunopathol.,17:425-439.Unsuckledspecificpathogenfreecalveswereinoculatedat3-4weeksofage,ei
3、therintranasally(IN)ororally(0)withbovinecoronavirusor0plusIN(O/IN)or0withbovinerotavirus.Sheddingofvirusinnasalorfecalsamples,andvirus-infectednasalepithelialcellsweredetectedusingimmunofluorescentstaining(IF),ELISAorimmuneelectronmicroscopy(IEM).Isotype-specificantibodytitersinsera,nasalandfeca
4、lsamplesweredeterminedbyELISA.Calvesinoculatedwithcoronavirusshedvirusinfecesandviruswasdetectedinnasalepithelialcells.NasalsheddingpersistedlongerinIN-inoculatedcalvesthaninO-inoculatedcalvesandlongerthanfecalsheddinginbothINandO-inoculatedcalves.Diarrheaoccurredinallcalves,buttherewerenosignsof
5、respiratorydisease.Calvesinoculatedwithrotavirushadsimilarpatternsofdiarrheaandfecalshedding,butgenerallyofshorterdurationthanincoronavirus-inoculatedcalves.Nonasalsheddingofrotaviruswasdetected.PeakIgManttbodyresponess,inmostcalves,weredetectedinfecalandnasalspecimentsat7-10dayspost-exposure(DPE
6、),preceedingpeakIgAresponseswhichoccurredat10-14DPE.Thenasalantibodyresponsesoccurredinallvirus-inoculatedcalvesevenintheabsenceofnasalsheddingofvirusinrotavirus-inoculatedcalves.CalvesinoculatedwithcoronavirushadhighertitersofIgMandIgAantibodiesinfecalandnasalsamplesthanrotavirus-inoculatedcalve
7、s.Inmostinoculatedcalves,maximaltitersofIgMorIgAantibodiescorrelatedwiththecessationoffecalornasalvirusshedding.AsimilarsequenceofappearanceofIgMandIgAantibodiesoccurredinserum,butIgAantibodiespersistedforashorterperio
