资源描述：
《冠状病毒Coronavirusl论文-1989 Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves_ Comparison w.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、VeterinaryMicrobiology,19(1989)217-231217ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsDevelopmentofProteinA-GoldImmunoelectronMicroscopyforDetectionofBovineCoronavirusinCalves:ComparisonwithELISAandDirectImmunofluorescenceofNasalEpithelialCells*R.A.HE
2、CKERT,L.J.SAIFandG.W.MYERSFoodAnimalHealthResearchProgram,OhioAgriculturalResearchandDevelopmentCenter,TheOhioStateUniversity,Wooster,0H44691(U.S.A.)(Acceptedforpublication17October1988)ABSTRACTHeckert,R.A.,Saif,L.J.andMyers,G.W.,1989.DevelopmentofproteinA-goldimmunoele
3、ctronmicroscopyfordetectionofbovinecoronavirusincalves:comparisonwithELISAanddirectimmunofluorescenceofnasalepithelialcells.Vet.Microbiol.,19:217-231.AproteinA-colloidalgoldimmunoelectronmicroscopy(PAG-IEM)techniquewasdevelopedforthedetectionofbovinecoronavirus(BCV)inth
4、efecesandnasalsecretionsofinfectedcalves.Fecesornasalswabfluidswereincubatedsequentiallywithhyperimmunebovineanti-bovinecoronavirusserumandproteinA-gold,negativelystained,appliedtoformvar-coatedcoppergridsandviewedusinganelectronmicroscope.ThePAG-IEMmethodspecificallyid
5、entifiedBCVparticlesandpossiblesubviralparticlesinfecesandnasal-swabfluidsfrominfectedcalves.ThePAG-IEMmethoddidnotlabelotherenvelopedentericvirusesormorphologicallysimilarfringedparticlescommonlyfoundinfeces.DetectionofBCVusingPAG-IEMwascomparedwithELISAanddirectimmuno
6、fluorescence(IF)ofnasalepithelialcellsbymonitoringfecalandrespiratorytractsheddingofBCVfromtwoexperimentallyinfectedandtwonaturallyinfectedcalvesfrombirthto3weeksofage.PAG-IEMandELISAdetectedsheddingofBCVinfecal(4/4animals)andnasal(3/4animals)samplesforanaverageof5.25da
7、yseach.TheobservedagreementofBCVdetectionbyPAG-IEMandELISAwas85%.PAG-IEMmaybeamoresensitiveimmu-noassayforthedetectionofBCVindiagnosticspecimensfrominfectedneonatalcalvesthanELISA.BCVinfectionofnasalepithelialcellswasdetectedbyimmunofluorescencein4/4calves,persistedfort
8、hedurationofthestudyin2/4calvesandwassporadicintheothertwoanimals.TheobservedagreementofBCVdetectionbyPAG-IEMa
