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1、JournalofVirologicalMethods,19(1988)215-224215ElsevierJVM00694EvaluationofanimmunogoldelectronmicroscopytechniquefordetectingbovinecoronavirusA.A.El-Ghorr,D.R.SnodgrassandF.M.M.ScottMoredunResearchInstitute,Edinburgh,Scotland,U.K.(Accepted15December1987)SummaryAsolidphasecolloidalgoldimmunoele
2、ctronmicroscopy(IGEM)techniquefordetectingbovinecoronavirus(BCV)wasdevelopedandshowntobespecific.Thistestwascomparedwiththreeotherdiagnostictestsusingfifteenfaecalsamples.Bovinecoronaviruswasdetectedin2samplesbydirectelectronmicroscopy(DEM),in3samplesbyimmunosorbentelectronmicroscopy,in5sample
3、sbyhaemadsorption-elution-haemagglutinationandin6samplesbyIGEM.NinetyfourfaecalsamplesweretestedbyDEMandIGEM.Of26samplesfoundtocontainBCVbyIGEMonly14werepositivebyDEM.TheIGEMtechniqueissimple,efficientandlesssusceptiblethanotherstonon-specificreactions.Bovinecoronavirus;Immunogold;Electronmicr
4、oscopy,DiagnosisIntroductionNeonatalcalfdiarrhoeaisasyndromeofcomplexaetiologyinwhichseveralin-fectiousagentsinteractwithenvironmentalinfluencestodeterminethehealthoftheyoungcalf.Bovinecoronavirus(BCV)isanendemicvirusknowntobein-volvedincalfdiarrhoea(Bridgeretal.,1978;Mebusetal.,1973;Pate1etal
5、.,1982;Reynoldsetal.,1986;Saifetal.,1986;Snodgrassetal.,1986;Stairetal.,1972),andhasbeenisolatedalsofromtherespiratorytract(McNultyetal.,1984;Saifetal.,1986).IntworecentstudiesBCVwasdetectedin4%of302diarrhoeiccalvesinScotlandandthenorthofEngland(Snodgrassetal.,1986)and14%of490diar-rhoeiccalves
6、inthesouthofEnglandandWales(Reynoldsetal.,1986).Correspondenceto:D.R.Snodgrass,MoredunResearchInstitute,408GilmertonRoad,Edinburgh,EH177JH,Scotland,U.K.0166-0934/88/$03.5001988ElsevierSciencePublishersB.V.(BiomedicalDivision)216SeveralmethodshavebeenusedtodetectBCVinfaeces.Withdirectelectronmi
7、croscopy(DEM)(Reynoldsetal.,1984)itisoftendifficulttodifferentiatepleo-morphiccoronavirusparticlesfrommembranousmaterialorotherfringedbodies.Thecharacteristicmorphologyofthecorevirionsurroundedbyafringeofpe-plomersisnotalwaysapparent(De
