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1、VeterinaryMicrobiology,23(1990)237-243237ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsIntestinalreplicationofaporcinerespiratorycoronaviruscloselyrelatedantigenicallytotheenterictransmissiblegastroenteritisvirusE.Cox,M.B.Pensaert,P.CallebautandK.vanDeunLaboratoryofVirology,Faculty
2、ofVeterinaryMedicine,StateUniversityofGent,Casinoplein24,B-9000Gent(Belgium)ABSTRACTCox,E.,Pensaert,M.B.,Callebaut,P.andvanDeun,K.,1990.Intestinalreplicationofaporcinerespiratorycoronaviruscloselyrelatedantigenicallytotransmissiblegastroenteritisvirus.Vet.Mi-crobiol.,23:237-243.One-week-oldpigletswe
3、reinoculatedwiththeporcinerespiratorycoronavirus(PRCV)eitherin-travenouslyordirectlyintothelumenofthegastrointestinaltract.Bothinoculationroutesresultedintheisolationofvirusfromthecaudalsmallintestine.Viralreplication,however,wasonlyobserveduponinoculationintothedigestivetractinquantitiesof>103TCIDs
4、o.Replicationremainedlimitedtoafewunidentifiedcellslocatedinorunderneaththeepitheliallayeratvillus-orcrypt-sites.Viruswasexcretedinthefaecesforseveraldaysbutinfectionoftherespiratorytractoccurredrarelyinthesamepigs.TheresultsofthisstudyindicatethatsmallchangesinmolecularstructurebetweenPRCVandtransm
5、issiblegastroenteritisvirushaveresultedinimportantchangesinhostcelltropism.INTRODUCTIONTransmissiblegastroenteritisvirus(TGEV)wasfirstisolatedbyDoyleandHutchings(1946).Itisanenteropathogeniccoronaviruswhichreplicatesinvillusepithelialcellsofthesmallintestine.Villusenterocytesbecomein-fected,degenera
6、teanddesquamatewhichresultsinatrophyofvilliandinseverewaterydiarrhoea.TGEVcaninducediarrhoeainswineofallageswithamortalityofnear100%inpigletsuntiltheageof2weeks(HooperandHaelterman,1969).Between12and24%ofsowspossessedseroneutralizing(SN)antibodiesagainstTGEVinserologicalsurveyscarriedoutamongtheBelg
7、iansowpop-ulationbetween1969and1984.In1984,therewasanunexpectedlyhighin-cidenceof68%animalswithTGEV-SNantibodies.Sincetherehadbeennoincreasedincidenceofdiarrhoeaandsincevaccinationwasnotperformed0378-
