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1、J.gen.Virol.(1983),64,127-133.PrintedinGreatBritain127Keywords:mousehepatitisvirus/cDNAprobes/subgenomicRNA/straincomparisonCharacterizationofMurineCoronavirusRNAbyHybridizationwithVirus-specificcDNAProbesBySUSANR.WEISS~*ANDJULIANL.LEIBOWITZ21DepartmentofMicrobiologyG/2,U
2、niversityofPennsylvania,Philadelphia,Pennsylvania19104and2DepartmentofPathology,UniversityofCalifornia,SanDiegoSchoolofMedicine,LaJolla,California92093,U.S.A.(Accepted24August1982)SUMMARYGenomeRNAofmousehepatitisvirus(MHV)strainA59hasbeenusedasatemplatetosynthesizetwoviru
3、s-specificprobes:cDNA~ep,representingthemajorityofsequencesofthegenomeRNAandcDNAy,representingthe3'endofthegenomeRNA.MolecularhybridizationwiththesecDNAswasusedtocharacterizebothgenomeRNAandintracellularvirus-specificRNAs.HybridizationofgenomeRNAsofMHVstrainsA59,JHM,andMH
4、V-3withA59cDNArepshowedthat,althoughthesethreestrainsexhibitdifferentpathogenicities,theycontaincloselyrelatednucleotidesequences.HybridizationofintracellularRNAfromMHV-infectedcellswithvirus-specificcDNAshowsthat(i)themajorityofvirus-specificRNAispolyadenylated,(ii)virus
5、-specificintracellularRNAcontainssixsubgenomicspeciesofthesamepolarityasgenomeRNAand(iii)allsubgenomicRNAscontainthesameYsequencesasthegenomeRNAandthusformanestedsetofRNAs.INTRODUCTIONCoronavirusescauseacuteand/orpersistentdiseaseinmanyspeciesofanimals.Onefactorthatdeterm
6、inesthetargetorganandlethalityofcoronavirusinfectionisthestrainofvirus(McIntosh,1974).Forexample,moststrainsofmousehepatitisvirus(MHV),suchasMHV-3,wereisolatedfromtheliversofinfectedmiceandareprimarilyhepatotropic(McIntosh,1974).TheA59strain,whichiscommonlyusedinthelabora
7、torybecauseitgrowstohightitresincellculture,isonlyweaklypathogenic(Robb&Bond,1979).TheJHMstrainisneurotropicandcausespersistentdemyelinatingdisease(Baileyetal.,1949).Infectionofrodentswiththisstrainhasbeencitedasamodelformultiplesclerosis.MostMHVstrainsarecloselyrelatedse
8、rologically(McIntosh,1974).Biochemicalstudieshavebeencarriedouttomeasurethenucleotidehomologiesa
