产漆酶裂褶菌筛选和其产酶培养条件优化探究

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1、产漆酶裂褶菌筛选和其产酶培养条件优化探究　　摘要：从采自秦岭的森林腐木菌苔上分离到一株产漆酶的裂褶菌（SchizophyllumcommuneFr.）菌株mys005，对其产漆酶培养方式、培养基组成及培养条件进行了优化试验。结果表明，裂褶菌菌株mys005的产漆酶适宜培养方式为液体浅层振荡培养，含有Cu2+、Mn2+、Zn2+的微量元素复合液、洋葱（AlliumcepaL.）、麦（TriticumaestivumL.）麸对其产漆酶都有较大的促进作用，而愈创木酚则对其生长和产漆酶有抑制作用。由新鲜马铃薯（SolanumtuberosumL.）200g、K2HPO4

2、1.00g、NaCl0.50g、MgSO4·7H2O0.50g、NaNO32.50g、CaCl2·2H2O0.10g、FeCl30.02g、吐温-801.20mL溶于1000mL去离子水中组成的培养基在添加微量元素复合液（50mg/mLCuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mL、洋葱100g、麦麸10g后，并且培养基起始pH4.5、培养温度30℃、培养摇床转速200r/min、培养时间7d，则摇瓶发酵的漆酶产酶量最高可达1121U/mL，显示出了良好的工业开发潜力。关键词：漆酶；裂褶菌（Schizophyl

3、lumcommuneFr.）；筛选；产酶培养基；优化20中图分类号：Q554+.9；S646.1+9；Q93-33文献标识码：A文章编号：0439-8114（2013）12-2792-06IsolationofSchizophyllumcommuneProducingLaccaseandOptimizationofLaccaseProductionConditionsZHAOWen-juan，XUSheng-yun，RENPing（EnzymeEngineeringInstituteofShaanxiSciences/ShaanxiEngineeringCent

4、erforEnzymeTechnology，Xi’an710600，China）Abstract：ASchizophyllumcommuneFr.strainmys005producinglaccasewasisolatedfromtherottenwoodlawninforestofQinlin.Theculturemethodforlaccaseproduction，mediumcompositionandcultivationconditionswasoptimized.Resultsshowedthatthesuitablecultivationmeth

5、odforS.communestrainmys005toproducelaccasewasshallowliquidshakingculture.TraceelementscompoundliquidcontainingCu2+，Mn2+，Zn2+，onion（AlliumcepaL.），wheat（TriticumaestivumL.）brancouldpromotelaccaseproduction；whileguaiacolcouldinhibitthegrowthandlaccaseproduction.Ifaddingtraceelementcompo

6、undsolution（50mg/mL20CuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mL，onion100g，wheatbran10g，inthemediumcontainingfreshpotato（SolanumtuberosumL.）200g，K2HPO41.00g，NaCl0.50g，MgSO4·7H2O0.50g，NaNO32.50g，CaCl2·2H2O0.10g、FeCl30.02g、tween-801.20mLin1000mLdeionizedwater，andkeepingthestartin

7、gpHat4.5，startingtemperatureat30℃，cultureshakerspeedat200r/min，culturingfor7d，thelaccaseenzymeproductionyieldinshakeflaskfermentationcouldbeuptoamaximumof1121U/mL，showinggreatpotentialforindustrialdevelopment.Keywords：laccase；SchizophyllumcommuneFr.；isolation；enzymeproducingmedium；op

8、timization漆酶