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1、氟伐他汀对博来霉素A5致大鼠肺纤维化的治疗作用作者：赵峰戚好文赵一玲吕荣【关键词】肺纤维化关键词：肺纤维化;普伐他丁;博来霉索A5摘要：冃的观察氟伐他汀(fluvastatin)对博来霉素(bleomycinA5)所致的大鼠肺纤维化的治疗作用，为肺纤维化的治疗探索一种新的方法.方法雄性SD大鼠30只，随机分为正常对照组(C组)、博来霉素组(B组)及根据给博来霉素后第1,15FI始每H按20mgkg-1及10mgkg-1给予氟伐他汀灌胃治疗分为4个治疗组(TH1,TL1,TH15及TL15组)・28d后处死所有动物，提取肺组织，HE染色，显微镜下观
2、察，LeicaQ500MC图像分析系统测定肺泡间隔成纤维细胞数量、厚度及肺间质面积.Masson^苦味酸天狼猩红染色，观察基质胶原及I,III型胶原变化•肺组织匀浆测定耗脯氨酸含量，并比较各组间变化.结果①B组的肺泡间隔成纤维细胞数量、厚度、肺间质面积及肺组织轻脯氨酸含量均高于C组(P<0.01),各氟伐他汀治疗组的以上各观察指标均低于B组(P<；0.01或P≪0.05)•②肺泡间隔成纤维细胞数量、厚度、肺间质面积在TH1组低于TH15组(P<O.01),TL1组低于TL15组(P<O・01或P<O.05)•③B
3、组基质胶原及I,III型胶原分布较C组明显增多，各氟伐他汀治疗组均有不同程度的减少.结论氟伐他汀对博來霉索所致的大鼠肺纤维化具有明显治疗作用，且早期治疗较晩期治疗效果更为明显・Keywords:pulmonaryfibrosis;pravastatin;bleomycinA5Abstract:AIMToinvestigatethetherapeuticeffectofflu-vastatininbleomycin-inducedpulmonaryfibrosisofrats・METHODSThirtySDratsweredividedintosi
4、xgroups:Thenormalcontrolgroup(GroupC),themodelgrouptreat-edwithbleomycinA5(GroupB)andtherest(GroupsTH1,TH15,TLlandTL15)sonamedaccordingtothedoseofflu-vastatinwhichwere20mgkg-1andlOmgkg-1andthedatewhichwerethefirstandthefifteenlhdayaftertheratsweregivenbleomycinA5respectively.
5、Alltheratswerekilledonthetwenty-eighthday-Thenumberoffibroblastandthethicknessofalveolarwal1,theareaofmesenchyinainlungtissueweremeasuredbyHEstaining.Theextracellularma-trixandI,IIItypecollageninlungtissuewereobservedbyMassonandsiriusredstainingandhydroxyprolinewasmea-suredin
6、lungtissuehomogenates.RESULTSThenumberoffibroblastandthethicknessoflungalveolarwal1,theareaofmesenchymaandthecontentofhydroxyprolineinlungtis-sueweresignificantlyhigherinGroupBthaninGroupC(P<0.01)•ThedatainthetreatmentgroupswerelowerthanthoseinGroupB(P<0.OlorP<0.05).
7、ItwasalsofoundthatthedataofHEstainingwerelowerinGroupTHIthaninGroupTH15(P<0.01)andinGroupTLlthaninGroupTL15(P&It;0.OlorP&It;0.05).Inaddition,theextracellularmatrixandI,IlltypecollageninlungtissueweremoreingroupBthaningroupC,andcomparedwithgroupB,theywerelessinthetreatment,
8、groups.CONCLUSIONFluvas-tatinmightbeatherapeuticmedicineforpulmonary
