没药甾酮对胆管癌 RBE 细胞移植瘤生长和 miRNA表达谱的影响.pdf

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1、．640．安徽医药AnhuiMedicalandPharmaceuticalJournal2015Apr；19(4)没药甾酮对胆管癌RBE细胞移植瘤生长和miRNA表达谱的影响仲飞，童铸廷，范璐璐，姚梦群，孙国平(安徽医科大学第一附属医院，安徽合肥230022)摘要：目的研究没药甾酮对胆管癌RBE细胞株移植瘤生长和miRNA表达的影响。方法雌性SD大鼠建立胆管癌RBE细胞株移植瘤模型，随机分为对照组和没药甾酮高、低剂量实验组；高、低剂量实验组大鼠分别予40、20mg·kg～·d没药甾酮灌胃，对照组

2、大鼠予生理盐水灌胃。每5天测量裸鼠质量和肿瘤体积，处理30d后取材，提取肿瘤组织总RNA并分离miR—NA。采用微阵列杂交，通过芯片扫描和数据分析获得miRNAs表达谱。结果高、低剂量实验组大鼠移植瘤体积分别在处理20d和25d后显著低于对照组(P<0．01)；处理30d后高、低剂量组移植瘤质量均显著低于对照组(P<0．01)。芯片结果显示，高剂量实验组瘤组织miRNAs表达谱与对照组相比有显著差异。结论没药甾酮可以显著抑制胆管癌RBE细胞株移植瘤生长并影响其miRNA的表达。关键词：没药甾酮；胆

3、管癌；移植瘤；microRNAsdoi：10．3969／j．issn．1009—6469．2015．04．007EfectsofguggulsteroneongrowthandmicroRNAsexpressionprofileintransplantedtumorofcholangiocarcinomaRBEcellsZHONGFei，TONGZhu—ring，FANLu—lu，etal(DepartmentofOncology，FintAffiliatedHospital，AnhuiMedic

4、alUniversity，Hefei230022，China)AbstractObjectiveTodeterminetheeffectsofguggulsteroneonthegro~handmicroRNAsexpressionprofileintransplantedtumorofhumancholangiocarcinomaRBEcells．MethodsFemaleSDnudemicewereinjectedwithRBEcellstoestablishtransplantedtumor

5、models．Themicewereequallyrandomizedinto3groupsincludingcontrolgroup，highdoseandlowdosetreatmentgroups．Oraladmin—istrationofguggulsterane[0，40and20mg·kg一·drespectively]weregiventothecorrespondingmicein72haftertransplanta—tion．Tumorvolumesandweightswere

6、measuredevery5d．Miceweresacrificedafter30days’treatment．TotalRNAwasextractedandmiRNAswereisolatedandhybridizedwithmiRNAsmicroarrays．ResultsThegro~hoftransplantedtumo~wassignificantlysup—pressedintreatmentgroups．Tumorsvolumeweresignificantlysmallerinhi

7、ghdosegroupandlowdosegroupthaninthecontrolafter20days’(P<0．01)and25days’treatment(P<0．01)．ThemicroarrayassayshowedsignificantlydifferentmiRNAsexpressionintumorsofhi【ghdosegroupcomparedwiththecontrolgroupConclusionGuggulsteronesignificantlysuppressesth

8、egrowthoftransplan·tedtumorofhumanch0lanocarcinomaRBEcellsandaffectmicroRNAsexpressionprofile．Keywords：guggulsterone；cholangiocarcinoma；transplantedtumor；microRNAs小分子RNA(microRNA，miRNA)是一类广泛左右的非编码单链小分子RNA，它们通过介导转录存在于动物和植物等多细胞生物中约22个核苷酸后基因沉默，在生