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1、3,6-二羟黄酮对乳腺癌细胞MDA-MB-453移植瘤生长和miRNAs表达谱的影响谢艳，常徽，舒芙蓉，赵长君，糜漫天（400038重庆，第三军医大学军事预防医学院营养与食品卫生学教研室，重庆市医学营养研究中心，重庆市营养与食品安全重点实验室）[摘要]目的观察3,6-二羟黄酮对HER-2/neu高表达人乳腺癌细胞株MDA-MB-453移植瘤生长和miRNA表达谱的影响。方法以18只雌性BALB/c裸鼠建立乳腺癌MDA-MB-453细胞移植瘤模型，按照完全随机设计原则平均分入对照组和高、低剂量作用组3组，移植后72h开始给予各处理因素，低、高
2、剂量组分别以10和20mg/（kg·d）的3,6-二羟黄酮灌胃，对照组以等量的生理盐水灌胃。每4天测量1次肿瘤体积和裸鼠体质量，处理28d后取材，提取肿瘤组织总RNA并分离miRNA，样品采用PolyAPolymerase加尾后利用T4RNA连接酶将带有生物素标记的信号分子与之连接，进而与微阵列杂交，通过芯片扫描和数据分析获得miRNAs表达谱。结果高、低剂量组移植瘤生长速度低于对照组，肿瘤体积分别在处理12d和16d后显著低于对照组(P＜0.05)；处理24d后高、低剂量组移植瘤质量均显著低于对照组(P＜0.01)。miRNAs表达谱分析
3、显示，高剂量组瘤组织miRNAs表达与对照组相比显著上调的有12个，显著下降的有15个。结论3,6-二羟黄酮可显著抑制人乳腺癌细胞株MDA-MB-453移植瘤的生长并影响其miRNAs表达谱。[关键词]3,6-二羟黄酮；乳腺癌；移植瘤；microRNAs[中图法分类号][文献标志码]AEffectsof3,6-dihydroxyflavoneonthegrowthandmicroRNAsexpressionprofileoftransplantedtumorofbreastcancerMDA-MB-453cells[Abstract]Obj
4、ectiveToinvestigatetheeffectsof3,6-dihydroxyflavoneonthegrowthandmicroRNAsexpressionprofileoftransplantedtumorofhumanbreastcancerMDA-MB-453cellsover-expressionHER-2/neu.MethodsEighteenfemaleBALB/cnudemicewereinjectedwithMDA-MB-453cellstoestablishtransplantedtumormodels.The
5、micewereequallyrandomizedinto3groupsincludingcontrolgroup,lowdoseandhighdosetreatmentgroups.Oraladministrationof3,6-dihydroxyflavone[0,10and20mg/（kg·d）respectively]tothemicebearingcancercellxenograftsafter72h.Tumorvolumesweremeasuredeveryfourdays.Miceweresacrificedafter28d
6、aystreatment,totalRNAwasextractedandmiRNAswereisolated.Sampleswerethenlinkedwithbiotin-labeledsignalingmolecules,andwerehybridizedwithmiRNAsmicroarrays.ResultsThegrowthoftransplantedtumorsintreatmentgroupsweresuppressed.Tumorsinhighdosegroupandlowdosegroupweresignificantly
7、smallerthanthecontrolafter12days(P＜0.05)and24daystreatmentrespectively(P＜0.01).Themicroarrayassayshowed12miRNAsexpressionintumorsofhighdosegroupweresignificantlyup-regulated,and15miRNAsexpressionweremarkedlydecreased.Conclusion3,6-Dihydroxyflavonecouldsignificantlysuppress
8、thegrowthoftransplantedtumorofhumanbreastcancerMDA-MB-453cells,andaffectitsmicroRNAsexpre
