家兔脑死亡后肝损伤机制-论文.pdf

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1、2013年1O月第2卷第5期ChinJHe·基础研究·家兔脑死亡后肝损伤机制钟自彪叶放发范晓礼李玲王彦峰【摘要】目的探讨家兔脑死亡后肝损伤机制。方法健康雄性新西兰家兔60只，按随机数字表法随机分为脑死亡组和假手术组，每组30只。脑死亡组采用颅骨钻孔置管，缓慢间断颅内加压至脑死亡；假手术组颅骨钻孔置管，不加压。两组分别于术后2、6、8h各处死10只，留取血标本及肝组织标本，采用比色法检测血清肝功能指标，苏木素．伊红(HE)染色观察肝脏的形态学改变，酶联免疫吸附试验(ELISA)检测血清中白介素(IL)．1B、肿瘤坏死因子(TNF)．的水平。两组间

2、数据比较采用t检验，组内各时间点比较采用方差分析及LSD．t检验。结果脑死亡组家兔术后8h的血清丙氨酸转氨酶(ALT)为(83+7)U／L，较术后6h的(52_+4)U／L明显升高(LSD．t=29．65，P

3、血清TNF．为(35．3+4．0)mg／L、(43．4±6．9)mgL、(49．3±5．4)mg／L，均较对应假手术组的(25-4±3．1)mg／L、(24．2±7．1)mg／L、(25．4±2．0)mg／L明显升高，差异有统计学意义(=3．409，3．369，7．224；P

4、smofliverinjuryofrabbitsafterbraindeathZHONGZi—biao，YEQi扣，FANXiao—li，LlLing,WANGYan-feng．InstituteofHepatobiliaryDiseasesofWuhanUniversity,ZhongnanHospitalofWuhanUniversity,TransplantMedicalCenterofWuhanUniversity,Wuhan430071，ChinaCorrespondingauthor：YEQi-fa,Email：yqf_china@

5、163．com【Abstract】ObjectiveToexplorethemechanismofliverinjuryofrabbitsafterbraindeath．MethodsSixtyhealthymaleNewZealandrabbitswererandomlydividedintobraindeathgroup(n=30)andshamgroup(n=30)accordingtotherandomnumbertable．Rabbitsinbraindeathgroupwereputtobraindeathbycranialdril

6、ling，eatheteringandincreasingintracranialpressureinaslow，intermittentway．Whilerabbitsinshamgroupunderwentcranialdrillingandcatheteringwithoutpressuring．Tenrabbitsfromeachgroupwererespectivelyputtodeathatthetimepointsof2，6，8hafteroperation，andthesamplesofbloodandlivertissuesw

7、erecollected．Serumindexesofliverfunctionweredetectedbycolorimetry．Morphologychangeofliverwasobservedbyhaematoxylin—eosin(HE)staining．Thelevelsofinterleukin(IL)一1pandtumornecrosisfactor(TNF)一仪weredetectedbyenzymelinkedimmunosorbentassay(ELISA)．Thedifferencebetweentwogroupswas

8、comparedusingttestandthedifferenceofeachtimepointswithinthegroupwascompared