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1、吉林农业大学学报2011,33(6):649~653http://xuebao.jlau.edu.cnJournalofJilinAgriculturalUniversityE-mail:jlndxb@vip.sina.com光照和激素对耐热型大白菜游离小孢子培养的X影响121XX11盛鹏,张秀荣,岳艳玲,李晶晶,邓静1.云南农业大学园林园艺学院,昆明650201;2.松原市前郭县白依拉嘎乡农业技术推广站,前郭131113摘要:将分离纯化的小孢子悬浮培养在添加0,011,012,013,015,110mg/L的NAA和0,0105,01
2、1,012,013,015,110mg/L6-BA的NLN培养基中,在A(3000lx光培养)、B(10d暗培养再3000lx光培养)、C(15d暗培养再3000lx光培养)、D(ck,暗培养)条件下进行培养,研究光照和激素对耐热型大白菜/夏将军0游离小孢子成胚及胚状体发育的影响。结果表明:011mg/L6-BA能显著促进胚状体的发生,使胚产量达到最高,为206个。同时附加012mg/LNAA不但保证有较高的胚产量(205个),还可促进胚状体发育,子叶胚及萌发胚可达3317%,畸形胚比例下降到1411%;暗培养能促进成胚,D处理成胚量高
3、,达149个,光培养则明显抑制成胚,但可促进胚状体发育,提高成苗率,其中以B处理成胚成苗效果较好,成胚108个,成苗率145%。关键词:耐热型大白菜;游离小孢子;光照;激素;成胚;成苗中图分类号:S63411文献标识码:A文章编号:1000-5684(2011)06-0649-05DOI:CNKI:22-1100/S.20110707.1624.003网络出版地址:http://www.cnki.net/kcms/detail/22.1100.S.20110707.1624.003.html引文格式:盛鹏,张秀荣,岳艳玲,等1光照和激素
4、对耐热型大白菜游离小孢子培养的影响[J].吉林农业大学学报,2011,33(6):649-653.EffectofDifferentLightandHormoneTreatmentsonIsolatedM-icrosporeCulturesofHeat-ToleranceChineseCabbage12111SHENGPeng,ZHANGXiu-rong,YUEYan-ling,LIJing-jing,DENGJing1.CollegeofLandscapeandHorticulture,YunnanAgriculturalUniver
5、sity,Kunming650201,China;2.QianguoBaiyilagaTownAgro-TechExtensionStationofSongyuanCity,Qianguo131113,ChinaAbstract:0,011,012,013,015and110mg/LNAAand0,0105,011,012,013and015,110mg/L6-BAwereaddedtoNLNmedia,andA-3000lxlight,B-10ddarkthen3000lxlight,C-15ddarkthen3000lxlighta
6、ndD-darkwereappliedtoculturingisolatedmicrosporeofhea-ttoleranceChinesecabbage-Xiajiangjiuinordertostudytheeffectoflightandhormoneonthegrowthandinductionofmicrospore-derivedembryos.Theresultsshowed:011mg/L6-BAcouldimproveinductionofm-icrospor-ederivedembryos.Thehighestyi
7、eld,206embryos,wasobtained.Atthesametimeadding012mg/LNAAcouldnotonlyinducehigherembryos,205embryos,butalsopromotegrowthofem-bryos.CotylednaryandGerminatedembryoincreasedto3317%andabnormalembryodecreasedto1411%.Darkculturecouldimproveinductionofmicrospore-derivedembryosan
8、dDtreatobtained149X基金项目:云南省自然科学基金项目(2006C0032Q)作者简介:盛鹏,男,硕士,从事蔬菜遗传育种工作。收稿日期:2010-05-31网络出版时间:2011-07-07
