氧化低密度脂蛋白对人脐静脉内皮细胞connexin40表达的影响

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1、脑血管病防治)2010年10月第鲞箜·349··基础研究·氧化低密度脂蛋白对人脐静脉内皮细胞Connexin40表达的影响郭士遵，章顺荣，王宁夫，洪鸣呜，高越，王晓楠，封菲(杭州市第一人民医院，浙江杭州310006)中图分类号：R541．4文献标识码：A文章编号：1009—816X(2010)05—0349—05DOI：10．3969／j．issn．1009—816X．2010．05．09TheEfectofOxidizedLDLontheExpressionofConnexin40GeneinCulturedHuma

2、nUmbilicalVeinEndotheliumCel1．GUOShi一盈m，ZHANGShun-rong，WANGNing-fu，eta1．DepartmentofCardiology，FirstPeople’sHospitalofHangzhou，Zhejiang310006，China[Abstract]ObjectiveToinvestigatetheefectofOxidizedLDL(Ox-LDL)ontheexpressionofGapjunctionproteinConnexin40geneincul

3、turedhumanmnbilicalveinendotheliumcell(HUVEC)．MethodsHUVECwasintervenedwithdiferentconcentrationsofOX—LDL：HUVECculturedweredividedintocontrolgroup，andOx—LDL50mg／L，lOOmg／Land200mg／Linterventiongroups，24hoursafterbeinggivenoxidizedLDL，theexpressionofConnexindOgene

4、mRNAofculturedHUVECwasdetectedwithRT-PCR；Forinter—ventionwithsameconcentrationofOX—LDLbutdiferenttime，HUVECweredividedinto6groups，theyallwereintervenedwithOX—LDL50mg／Lbutintervenedfor0hour(controlgroup)，6hours，12hours，24hours，48hoursand72hours，respectively；while

5、theexpressionlevelofConnexin40proteinwasdetectedwithimmunoeytoehemistrymethodwhileHUVECwasintervenedwithOx-LDLOmg／LandOX—LDLlOOmg／L．ResultsDiferentconcentrations(50mg／L，lOOrog／L，200mg／L)ofOX-LDLcanup-regulatemRNAexpressionlevelofConnexin40geneinculturedHUVECafte

6、r24hoursintervention(P<0．O1)．InterventionwithOx—LDL50mg／Lfordiferenttimescanalup-regulatethemRNAexpressionlevelofculturedHUVEC(P<0．01)，amongthemthemostsignificantup—regI】1ationefectwasingroupsofinterventionfor6hoursand12hours(P<0．01)．Comparedwiththecontrolgroup，

7、theproteinexpressionlevelofConnexin40geneofculturedHUVECinthegroupintervenedwithOX—LDL100m#Lfor24hourswasmarkedlyup-regulated(P<0．01)．ConclusionsOx—LDLmayup-regulatetheproteinandmRNAexpressionlevelofConnexin40geneincul—turedHUVECwithinshor【time，indicatingthatCon

8、nexin40mayplayanimportantroleinthepro-atheroscleroticefectofOX—LDL．[KeywordsJGapjunctionprotein；Connexin40；OxidizedLDL；HumanveinendotheliumeeH；Geneexpression动脉粥样硬化(At