大豆GmGAMYB1启动子克隆与分析-论文.pdf

《大豆GmGAMYB1启动子克隆与分析-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、第32卷第2期大豆科学V01．32No．22013正4月S0YBEANSCIENCEApr．2013大豆GmGAMYB1启动子克隆与分析谷月娇，赵琳，张彦威，卢清瑶，宋仙萍，李文滨(大豆生物学教育部重点实验室／农业部东北大豆生物学与遗传育种重点实验室／东北农业大学大豆研究所，黑龙江哈尔滨150030)摘要：利用PCR技术从大豆品种东农42基因组中分离得到了GmGAMYB1基因启动子片段pGmGAMYB1，定向替换载体pBIl21的CAMV35S组成型启动子，构建表达载体pBI121一pGmGAMYB1，驱动下游报告基因GUS基因表达，并利用PLACE和PlantCARE在线

2、启动子预测工具进行分析。结果表明：序列中含有多种典型的光及各种激素和胁迫诱导特异性表达元件，如光应答元件如3-AFtbindingsite、BOX-4、T—Box和TCT—motif；赤霉素应答元件GARE；脱落酸应答元件ABRE；热激元件CCAATBox；节律钟Ciradian等。关键词：GmGAMYB1启动子；GUS基因；组织化学染色中图分类号：$565．1文献标识码：A文章编号：1000-9841(2013)02-0160-05IsolationandAnalysisofPromoterofGmGAMYB1fromSoybeanGUYue-jiao，ZHAOLin，Z

3、HANGYan—wei，LUQing—yao，SONGXian·ping，LIWen—bin(KeyLaboratoryofSoybeanBiologyinChineseMinistryofEducation／KeyLaboratoryofBiologyandGenetics＆BreedingibrSoybeaninNortheastChina．MinistryofAgriculture／SoybeanResearchIristituteofNortheastAculturalUniversity，Harbin150030，China)Abstract：Soybean(Gl

4、ycinemax,)isakindoftypicalplantinthedevelopmentalbiologyandmolecularbiologyresearch，thecompletionofsoybeangenomehasprovidedalotofconvenienceforresearch，butthereisstillSOlittleachievementonthesoy—beanhighlyeffectiveinduciblepromoters．Gibberellin(GA)biosynthesisandsignalingpathwayshavebeendi

5、scussedindetailinrecentyears．Inaddition，severalreviewsdiscussgeneralmechanismsofmorphogenesis．GAMYB1geneisanimportantpro·motivefactorinthesignaltransduetionpathwaysofGA．Inthisstudy，thepromoterofGmGAMYB1wasclonedfromthesoybeanDongnong42byPCR．ItwasdireetionallyreplacedCAMV35Sconstitutiveprom

6、oterofthevectorpBI121．Theexpressionvec—torofpBI121一pGmGAMYB1wasconstructedandtheexpressionofdownstreamreporterGUSgenewasdrived．TheanalysisonpromotersequencebyonlinepromoterforecastingtoolPLACEandPlantCAREshowedthatsequencecontainedavarietyofspecificexpressionelements，suchaslightandvarioush

7、ormonesandstress—inducedelements．GUShistochemicalstainingwasusedtoexploretheroleofthepromoterinplantgrowthregulation．ThisstudyhadlaidagoodfoundationforGmGAMYB1char—acteristicstemporalandspatialexpression．Keywords：GmGAMYB1promoter；GUSgene；Histoehemiealstaining赤