下肢缺血＂晚时间窗＂的血管细胞黏附分子-1超声分子成像

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1、医学杂志2010年4月第12卷第4期JUltrasoundinClinMed，April2010．：．·2l7··实验研究·下肢缺血“晚时间窗"的血管细胞黏附分子一1超声分子成像谢佳佳杨莉吴爵非胡广全蔡晶晶纪丽景宾建国刘莹宾建平摘要目的探讨应用携带血管细胞黏附分子一1(VCAM一1)分子探针的超声分子成像，评价下肢缺血“晚时间窗”炎症反应的可行性。方法l2只实验小鼠结扎一侧下肢股动脉制备缺血模型，另一侧股动脉为对照。术后第3天随机先后(间隔30min)经尾静脉注入携抗小鼠VCAM—l单抗的靶向微泡(MB)和携同型抗体的对照微泡(MB)，并于8rain后行对比超

2、声检查，测量双侧下肢的显影强度(VI)；最后进行双下肢病理学检查。结果对比超声图像显示：缺血下肢MB组可见明显的超声显影，VI值高达(18．4±2．9)U；而在缺血下肢MB组仅见轻度的超声显影，VI值为(6．9±1．2)u，两微泡组问比较差异有统计学意义(P<0．05)。两组微泡在非缺血下肢的VI值未见明显差异(P>0．05)。但无论MB还是MB微泡，其在缺血下肢的VI值均明显高于非缺血下肢相应的VI值。免疫组化显示：缺血下肢血管有大量的VCAM一1表达，而非缺血下肢未见VCAM一1表达。结论应用VCAM一1分子探针的超声分子成像可有效评价下肢缺血“晚时间窗”

3、的炎症反应。关键词下肢缺血；血管细胞黏附分子一1；超声分子成像；造影剂；靶向微泡[中图法分类号]R654．4；R445．1[文献标识码]ALatetimewindowassessmentofinflammationinhind-limbischemiabyultrasoundmolecularimagingwithmicrobubblestargetedtoVCAM—-1XIEJiajia，YANGLi，wUJuefei，HUGuangquan，CAIJingjing，J1Lijing，BINJianguo，LIUYing，BINJianpingDepartm

4、entofCardiovascular，NanfangHospital，SouthernMedicalUniversity，Gdangzhou510515，ChinaABSTRACTObjectiveToexplorethefeasibilityofassessmentofinflammationinhind—limbischemiabyultrasoundmolecularimaging(UMI)withmicrobubbles(MB)targetedtoVCAM一1inlatetimewindow．MethodsOnesidehind—limbischem

5、iawasinducedin12experimentalmicebyfemoralarteryligation，theothersidewascontrasted．Onpostoperativeday3，UMIofthehind·-limbwasperformedinallmiceat8minutesafterintravenousinjectionofeitherVCAM——1targetingmicrobubbles(MBv)orisotypecontrolmicrobubbles(MBc)atrandomwith30mininterval，andthev

6、ideointensity(VI)wasmeasured．Atlast，allhind—limbswereexaminedbypathology．ResultsContrastultrasonicimagingshowedthatVIoftheischemiahind—limbwassignificantlyhigher(P<0．05)forMBv(18．4±2．9)UcomparedwithMBc(6．9±1．2)U．However，therewasnosignificantdifferenceofVIinnon—ischemiahind—limbbetwe

7、enMBvandMBcgroup(P>0．05)，butnomatterMBvorMBc，VIinischemiahind—limbwassignificantlyhigherthanthatinnon—ischemiahind—limb．AmarkedendothelialVCAM一1expressionwasonlynotedinischemiahind—limbbutnotinnon—ischemiahind—limbbyimmunohistochemistry．ConclusionUM1withmicrobubblestargetedtoVCAM——1

8、caneffectivelyevalu