资源描述:
《冠状病毒Coronavirusl论文-1980 Epizootic diarrhoea of adult cattle associated with a coronavirus-like agent.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、VeterinaryMicrobiology,5(1980)151--154151©ElsevierScientificPublishingCompany,Amsterdam--PrintedinTheNetherlandsShortCommunicationEPIZOOTICDIARRHOEAOFADULTCATTLEASSOCIATEDWITHACORONAVIRUS-LIKEAGENTE.TAKAHASHI,Y.INABA,K.SATO,Y.ITO,H.KUROGI,H.AKASHI,K.SATODA,andT.OMORINationalInstituteofAnimalHeal
2、th,Kannondai,Tsukuba,Ibaraki(Japan)(Accepted13December1979)ABSTRACTTakahashi,E.,Inaba,Y.,Sato,K.,Ito,Y.,Kurogi,H.,Akashi,H.,Satoda,K.andOmori,T.,1980.Epizooticdiarrhoeaofadultcattleassociatedwithacoronavirus-likeagent.Vet.Microbiol.,5:151--154.AnepizooticofacutediarrhoeaofadultcattleoccurredinJa
3、panduringthewinterof1976to1977.Amajorityofadultcattleclinicallydiagnosedashavingthedisease,showedsignificantrisesinantibodytitrestobovinecoronavirus,whereasonlyasmallminorityshowedserologicalevidenceofrecentinfectionwithcalfrotavirus,bovineadenovirustype7,parainfluenzavirustype3,orbovineviraldia
4、rrhoea-mucosaldiseasevirus.Acoronavirus-likeagentwasdetectedbyelectron-microscopyinfaecalmaterialfromacowwithdiarrhoea,andwassubsequentlyisolatedinprimarybovinekidneycellcultures.INTRODUCTIONTheaetiologyofdiarrhoeainthebovineanimaliscomplex,andavarietyofviruseshavebeenassociatedwiththesyndrome.T
5、hepurposeofthepresentreportistogiveserologicalevidenceforanaetiologicalroleofbovinecoronavirusinanepizooticdiarrhoeaofadultcattleinJapan,withsupportiveelectron-microscopicalandcellculturestudies.MATERIALSANDMETHODSHerdsconcerned.IntenherdsfromcentralandwesternareasofJapan,150of200adultcowsdevelo
6、peddiarrhoeaduringthewinterof1976--1977.Milkproductiondeclinedbyanaverage20%duringtheoutbreakandfor3--4weeksduringrecoveryperiods.Serology.Pairedserumsamplesweretakenduringtheacuteandconvalescentstagesofthesyndrome,andweretestedforhaemaggiutination-inhibiting(HI)orneutralizing(NT)antibodiesagain
7、§treferencestrainsofbovinecoro-navirus(BVC)usingtechniquesdescribedbySharpeeetal.(1976)andStair152etal.(1972),ofcalfrotavirus(NCDV,Ferneliusetai.,1972),ofbovineadenovirustype7(BAV-7--Inabaetal.,1968),ofparainfluenzavirustype