冠状病毒Coronavirusl论文-1984 Preliminary observations on enteritis associated with a coronavirus-like agent in rabbits.pdf

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1、LaboratoryAnimals(1984)18,71-74.71Preliminaryobservationsonenteritisassociatedwithacoronavirus-likeagentinrabbitsP.EATONVersuchstierzuchtWIGAWilliGassnerGmbH,D-8741Sulzfeld,FederalRepublicofGermany"SummaryTable1.DistributionofmortalitybyageAnoutbreakoffatalenteritisin3-8-weekoldrabbi

2、tsinabarrier-maintainedbreedingcolonyisdescribed.ThelaboratoryfindingsandtreatmentsAge(weeks)%mortalityattemptedsuggestthataviralagentwastheprimary33,5pathogen.410·4527·5Aclosedbreedingcolonyoflaboratoryrabbits634·5(Chinchilla-BastardeandRussian)with1200does713-8washousedin2separateb

3、arrierroomsfor8years.88,6Duringthisperiodproductivityimprovedtogive91·7anaveragelittersizeof7·2andapre-weaningmortalityrateof12%.SevereentericdiseaseTherewasonlyrarelyavasculardisturbanceintheoccurredinIroominthespringof1979andserosaormucosalepitheliumoftheintestine,severalmonthslate

4、rinthe2ndroom.Routinealthoughpetechiaeweresometimesseeninthelaboratoryinvestigationsfailedtodemonstratethymus,Liver,kidney,spleenandlymphnodesanyknownpathogenbutelectronmicroscopyweremacroscopicallynormal.ThepHvaluesofrevealedacoronavirus-likeparticleintheintestinalintestinallumencon

5、tentswereasfollows:stomachcontentof94%ofthesamplesexamined.1·0-2'0;duodenum7'5;ileum7'0;caecum7,0-8,0.ClinicalpictureThecolonywashousedinmeshcages,fedonaLaboratoryinvestigationspelleteddietadlibitum(DeukaZuchtpellets:EndoparasitesDeutscheKraftfutterGmbH,WerkWorms,FRG)Smallnumbersofco

6、ccidialoocystswereregularlyandmaintainedat18±1°Cand45-60%RH.foundinfaecesexaminedbycentrifugationinaAnimalsweresickforupto24hpriortodeath.saturatedsaltsolution.SmearsfromscrapingsofTheywerereluctanttomove,hadastaringcoatthecaecalwallindeadrabbitsdidnotrevealandfrequentlyawetperineum.

7、Theabdomensignificantnumbersofoocysts.appearedslightlyswollen.Between40-60%wereaffectedandvirtuallyallclinicallysickanimalsBacteriadied.Deathsoccurredbetween3-8weeksofage.AerobicandanaerobiccultureofintestinalcontentsThedistributionofmortalitybyagewasasshownyieldedtherangeanddensityo

8、ffloraassoci